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Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II
In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-t...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890631/ https://www.ncbi.nlm.nih.gov/pubmed/26619013 http://dx.doi.org/10.1038/nmeth.3667 |
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| author | Sierra, Raymond G. Gati, Cornelius Laksmono, Hartawan Dao, E. Han Gul, Sheraz Fuller, Franklin Kern, Jan Chatterjee, Ruchira Ibrahim, Mohamed Brewster, Aaron S. Young, Iris D. Michels-Clark, Tara Aquila, Andrew Liang, Mengning Hunter, Mark S. Koglin, Jason E. Boutet, Sébastien Junco, Elia A. Hayes, Brandon Bogan, Michael J. Hampton, Christina Y. Puglisi, Elisabetta V. Sauter, Nicholas K. Stan, Claudiu A. Zouni, Athina Yano, Junko Yachandra, Vittal K. Soltis, S. Michael Puglisi, Joseph D. DeMirci, Hasan |
| author_facet | Sierra, Raymond G. Gati, Cornelius Laksmono, Hartawan Dao, E. Han Gul, Sheraz Fuller, Franklin Kern, Jan Chatterjee, Ruchira Ibrahim, Mohamed Brewster, Aaron S. Young, Iris D. Michels-Clark, Tara Aquila, Andrew Liang, Mengning Hunter, Mark S. Koglin, Jason E. Boutet, Sébastien Junco, Elia A. Hayes, Brandon Bogan, Michael J. Hampton, Christina Y. Puglisi, Elisabetta V. Sauter, Nicholas K. Stan, Claudiu A. Zouni, Athina Yano, Junko Yachandra, Vittal K. Soltis, S. Michael Puglisi, Joseph D. DeMirci, Hasan |
| author_sort | Sierra, Raymond G. |
| collection | PubMed |
| description | In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-temperature X-ray crystal structure of the 30S subunit bound to the antibiotic paromomycin was obtained in its native mother liquor. Compared to previous cryo-cooled structures, this new structure showed that paromomycin binds to the decoding center in a different conformation. |
| format | Online Article Text |
| id | pubmed-4890631 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-48906312016-07-01 Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II Sierra, Raymond G. Gati, Cornelius Laksmono, Hartawan Dao, E. Han Gul, Sheraz Fuller, Franklin Kern, Jan Chatterjee, Ruchira Ibrahim, Mohamed Brewster, Aaron S. Young, Iris D. Michels-Clark, Tara Aquila, Andrew Liang, Mengning Hunter, Mark S. Koglin, Jason E. Boutet, Sébastien Junco, Elia A. Hayes, Brandon Bogan, Michael J. Hampton, Christina Y. Puglisi, Elisabetta V. Sauter, Nicholas K. Stan, Claudiu A. Zouni, Athina Yano, Junko Yachandra, Vittal K. Soltis, S. Michael Puglisi, Joseph D. DeMirci, Hasan Nat Methods Article In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-temperature X-ray crystal structure of the 30S subunit bound to the antibiotic paromomycin was obtained in its native mother liquor. Compared to previous cryo-cooled structures, this new structure showed that paromomycin binds to the decoding center in a different conformation. 2015-11-30 2016-01 /pmc/articles/PMC4890631/ /pubmed/26619013 http://dx.doi.org/10.1038/nmeth.3667 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Sierra, Raymond G. Gati, Cornelius Laksmono, Hartawan Dao, E. Han Gul, Sheraz Fuller, Franklin Kern, Jan Chatterjee, Ruchira Ibrahim, Mohamed Brewster, Aaron S. Young, Iris D. Michels-Clark, Tara Aquila, Andrew Liang, Mengning Hunter, Mark S. Koglin, Jason E. Boutet, Sébastien Junco, Elia A. Hayes, Brandon Bogan, Michael J. Hampton, Christina Y. Puglisi, Elisabetta V. Sauter, Nicholas K. Stan, Claudiu A. Zouni, Athina Yano, Junko Yachandra, Vittal K. Soltis, S. Michael Puglisi, Joseph D. DeMirci, Hasan Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II |
| title | Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II |
| title_full | Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II |
| title_fullStr | Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II |
| title_full_unstemmed | Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II |
| title_short | Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II |
| title_sort | concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem-ii |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890631/ https://www.ncbi.nlm.nih.gov/pubmed/26619013 http://dx.doi.org/10.1038/nmeth.3667 |
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