Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells

Bacterial lipopolysaccharide (LPS) induces release of inflammatory mediators both in immune and epithelial cells. We investigated whether changes of epigenetic marks, including selected histone modification and DNA methylation, may drive or accompany the activation of COX-2 gene in HT-29 human intes...

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Autores principales: Angrisano, Tiziana, Pero, Raffaela, Brancaccio, Mariarita, Coretti, Lorena, Florio, Ermanno, Pezone, Antonio, Calabrò, Viola, Falco, Geppino, Keller, Simona, Lembo, Francesca, Avvedimento, Vittorio Enrico, Chiariotti, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890762/
https://www.ncbi.nlm.nih.gov/pubmed/27253528
http://dx.doi.org/10.1371/journal.pone.0156671
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author Angrisano, Tiziana
Pero, Raffaela
Brancaccio, Mariarita
Coretti, Lorena
Florio, Ermanno
Pezone, Antonio
Calabrò, Viola
Falco, Geppino
Keller, Simona
Lembo, Francesca
Avvedimento, Vittorio Enrico
Chiariotti, Lorenzo
author_facet Angrisano, Tiziana
Pero, Raffaela
Brancaccio, Mariarita
Coretti, Lorena
Florio, Ermanno
Pezone, Antonio
Calabrò, Viola
Falco, Geppino
Keller, Simona
Lembo, Francesca
Avvedimento, Vittorio Enrico
Chiariotti, Lorenzo
author_sort Angrisano, Tiziana
collection PubMed
description Bacterial lipopolysaccharide (LPS) induces release of inflammatory mediators both in immune and epithelial cells. We investigated whether changes of epigenetic marks, including selected histone modification and DNA methylation, may drive or accompany the activation of COX-2 gene in HT-29 human intestinal epithelial cells upon exposure to LPS. Here we describe cyclical histone acetylation (H3), methylation (H3K4, H3K9, H3K27) and DNA methylation changes occurring at COX-2 gene promoter overtime after LPS stimulation. Histone K27 methylation changes are carried out by the H3 demethylase JMJD3 and are essential for COX-2 induction by LPS. The changes of the histone code are associated with cyclical methylation signatures at the promoter and gene body of COX-2 gene.
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spelling pubmed-48907622016-06-10 Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells Angrisano, Tiziana Pero, Raffaela Brancaccio, Mariarita Coretti, Lorena Florio, Ermanno Pezone, Antonio Calabrò, Viola Falco, Geppino Keller, Simona Lembo, Francesca Avvedimento, Vittorio Enrico Chiariotti, Lorenzo PLoS One Research Article Bacterial lipopolysaccharide (LPS) induces release of inflammatory mediators both in immune and epithelial cells. We investigated whether changes of epigenetic marks, including selected histone modification and DNA methylation, may drive or accompany the activation of COX-2 gene in HT-29 human intestinal epithelial cells upon exposure to LPS. Here we describe cyclical histone acetylation (H3), methylation (H3K4, H3K9, H3K27) and DNA methylation changes occurring at COX-2 gene promoter overtime after LPS stimulation. Histone K27 methylation changes are carried out by the H3 demethylase JMJD3 and are essential for COX-2 induction by LPS. The changes of the histone code are associated with cyclical methylation signatures at the promoter and gene body of COX-2 gene. Public Library of Science 2016-06-02 /pmc/articles/PMC4890762/ /pubmed/27253528 http://dx.doi.org/10.1371/journal.pone.0156671 Text en © 2016 Angrisano et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Angrisano, Tiziana
Pero, Raffaela
Brancaccio, Mariarita
Coretti, Lorena
Florio, Ermanno
Pezone, Antonio
Calabrò, Viola
Falco, Geppino
Keller, Simona
Lembo, Francesca
Avvedimento, Vittorio Enrico
Chiariotti, Lorenzo
Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells
title Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells
title_full Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells
title_fullStr Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells
title_full_unstemmed Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells
title_short Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells
title_sort cyclical dna methylation and histone changes are induced by lps to activate cox-2 in human intestinal epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890762/
https://www.ncbi.nlm.nih.gov/pubmed/27253528
http://dx.doi.org/10.1371/journal.pone.0156671
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