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Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F(2) population of common bean evaluated by the straw test method. The DNA from 186 F(2) plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890856/ https://www.ncbi.nlm.nih.gov/pubmed/27335680 http://dx.doi.org/10.1155/2014/828102 |
Sumario: | The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F(2) population of common bean evaluated by the straw test method. The DNA from 186 F(2) plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F(2:4) progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F(2:4) progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance—13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection. |
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