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Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method

The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F(2) population of common bean evaluated by the straw test method. The DNA from 186 F(2) plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP...

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Autores principales: Lara, Letícia A. de C., dos Santos, João B., Veloso, Juliana S., Balestre, Marcio, Alves, Filipe C., Leite, Monik E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890856/
https://www.ncbi.nlm.nih.gov/pubmed/27335680
http://dx.doi.org/10.1155/2014/828102
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author Lara, Letícia A. de C.
dos Santos, João B.
Veloso, Juliana S.
Balestre, Marcio
Alves, Filipe C.
Leite, Monik E.
author_facet Lara, Letícia A. de C.
dos Santos, João B.
Veloso, Juliana S.
Balestre, Marcio
Alves, Filipe C.
Leite, Monik E.
author_sort Lara, Letícia A. de C.
collection PubMed
description The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F(2) population of common bean evaluated by the straw test method. The DNA from 186 F(2) plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F(2:4) progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F(2:4) progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance—13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection.
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spelling pubmed-48908562016-06-22 Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method Lara, Letícia A. de C. dos Santos, João B. Veloso, Juliana S. Balestre, Marcio Alves, Filipe C. Leite, Monik E. ISRN Mol Biol Research Article The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F(2) population of common bean evaluated by the straw test method. The DNA from 186 F(2) plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F(2:4) progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F(2:4) progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance—13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection. Hindawi Publishing Corporation 2014-02-06 /pmc/articles/PMC4890856/ /pubmed/27335680 http://dx.doi.org/10.1155/2014/828102 Text en Copyright © 2014 Letícia A. de C. Lara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lara, Letícia A. de C.
dos Santos, João B.
Veloso, Juliana S.
Balestre, Marcio
Alves, Filipe C.
Leite, Monik E.
Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
title Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
title_full Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
title_fullStr Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
title_full_unstemmed Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
title_short Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method
title_sort identification of qtls for resistance to sclerotinia sclerotiorum in carioca common bean by the moving away method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890856/
https://www.ncbi.nlm.nih.gov/pubmed/27335680
http://dx.doi.org/10.1155/2014/828102
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