Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis

Cutaneous leishmaniasis (CL) is an endemic disease and a public health problem in Hama governorate located in the central region of Syria. The aim of this study was to characterize Leishmania species isolated from human skin samples. A polymerase chain reaction, restriction fragment length polymorph...

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Detalles Bibliográficos
Autores principales: Al-Nahhas, Samar Anis, Kaldas, Rania Magdy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890916/
https://www.ncbi.nlm.nih.gov/pubmed/27335852
http://dx.doi.org/10.5402/2013/308726
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author Al-Nahhas, Samar Anis
Kaldas, Rania Magdy
author_facet Al-Nahhas, Samar Anis
Kaldas, Rania Magdy
author_sort Al-Nahhas, Samar Anis
collection PubMed
description Cutaneous leishmaniasis (CL) is an endemic disease and a public health problem in Hama governorate located in the central region of Syria. The aim of this study was to characterize Leishmania species isolated from human skin samples. A polymerase chain reaction, restriction fragment length polymorphism (PCR-RFLP) assay, was performed on skin lesion material samples from 32 patients with confirmed CL by direct microscopic examination in order to prove its usefulness and efficiency for identification of Leishmania species. Leishmania tropica (L. tropica) is confirmed as an etiologic agent of CL in this area.
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spelling pubmed-48909162016-06-22 Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis Al-Nahhas, Samar Anis Kaldas, Rania Magdy ISRN Parasitol Research Article Cutaneous leishmaniasis (CL) is an endemic disease and a public health problem in Hama governorate located in the central region of Syria. The aim of this study was to characterize Leishmania species isolated from human skin samples. A polymerase chain reaction, restriction fragment length polymorphism (PCR-RFLP) assay, was performed on skin lesion material samples from 32 patients with confirmed CL by direct microscopic examination in order to prove its usefulness and efficiency for identification of Leishmania species. Leishmania tropica (L. tropica) is confirmed as an etiologic agent of CL in this area. Hindawi Publishing Corporation 2013-09-19 /pmc/articles/PMC4890916/ /pubmed/27335852 http://dx.doi.org/10.5402/2013/308726 Text en Copyright © 2013 S. A. Al-Nahhas and R. M. Kaldas. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al-Nahhas, Samar Anis
Kaldas, Rania Magdy
Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis
title Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis
title_full Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis
title_fullStr Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis
title_full_unstemmed Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis
title_short Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis
title_sort characterization of leishmania species isolated from cutaneous human samples from central region of syria by rflp analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890916/
https://www.ncbi.nlm.nih.gov/pubmed/27335852
http://dx.doi.org/10.5402/2013/308726
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