Cargando…
Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To ident...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891005/ https://www.ncbi.nlm.nih.gov/pubmed/26848777 http://dx.doi.org/10.18632/oncotarget.7122 |
_version_ | 1782435199855886336 |
---|---|
author | Wang, Mengjie Dong, Qi Wang, Hua He, Yaqing Chen, Ying Zhang, Hong Wu, Rong Chen, Xinchun Zhou, Boping He, Jason Kung, Hsiang-Fu Huang, Canhua Wei, Yuquan Huang, Jian-dong Xu, Hongxi He, Ming-Liang |
author_facet | Wang, Mengjie Dong, Qi Wang, Hua He, Yaqing Chen, Ying Zhang, Hong Wu, Rong Chen, Xinchun Zhou, Boping He, Jason Kung, Hsiang-Fu Huang, Canhua Wei, Yuquan Huang, Jian-dong Xu, Hongxi He, Ming-Liang |
author_sort | Wang, Mengjie |
collection | PubMed |
description | There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection. |
format | Online Article Text |
id | pubmed-4891005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-48910052016-06-20 Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 Wang, Mengjie Dong, Qi Wang, Hua He, Yaqing Chen, Ying Zhang, Hong Wu, Rong Chen, Xinchun Zhou, Boping He, Jason Kung, Hsiang-Fu Huang, Canhua Wei, Yuquan Huang, Jian-dong Xu, Hongxi He, Ming-Liang Oncotarget Research Paper There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection. Impact Journals LLC 2016-02-01 /pmc/articles/PMC4891005/ /pubmed/26848777 http://dx.doi.org/10.18632/oncotarget.7122 Text en Copyright: © 2016 Wang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Mengjie Dong, Qi Wang, Hua He, Yaqing Chen, Ying Zhang, Hong Wu, Rong Chen, Xinchun Zhou, Boping He, Jason Kung, Hsiang-Fu Huang, Canhua Wei, Yuquan Huang, Jian-dong Xu, Hongxi He, Ming-Liang Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 |
title | Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 |
title_full | Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 |
title_fullStr | Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 |
title_full_unstemmed | Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 |
title_short | Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 |
title_sort | oblongifolin m, an active compound isolated from a chinese medical herb garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of erp57 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891005/ https://www.ncbi.nlm.nih.gov/pubmed/26848777 http://dx.doi.org/10.18632/oncotarget.7122 |
work_keys_str_mv | AT wangmengjie oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT dongqi oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT wanghua oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT heyaqing oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT chenying oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT zhanghong oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT wurong oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT chenxinchun oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT zhouboping oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT hejason oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT kunghsiangfu oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT huangcanhua oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT weiyuquan oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT huangjiandong oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT xuhongxi oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 AT hemingliang oblongifolinmanactivecompoundisolatedfromachinesemedicalherbgarciniaoblongifoliapotentlyinhibitsenterovirus71reproductionthroughdownregulationoferp57 |