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Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To ident...

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Autores principales: Wang, Mengjie, Dong, Qi, Wang, Hua, He, Yaqing, Chen, Ying, Zhang, Hong, Wu, Rong, Chen, Xinchun, Zhou, Boping, He, Jason, Kung, Hsiang-Fu, Huang, Canhua, Wei, Yuquan, Huang, Jian-dong, Xu, Hongxi, He, Ming-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891005/
https://www.ncbi.nlm.nih.gov/pubmed/26848777
http://dx.doi.org/10.18632/oncotarget.7122
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author Wang, Mengjie
Dong, Qi
Wang, Hua
He, Yaqing
Chen, Ying
Zhang, Hong
Wu, Rong
Chen, Xinchun
Zhou, Boping
He, Jason
Kung, Hsiang-Fu
Huang, Canhua
Wei, Yuquan
Huang, Jian-dong
Xu, Hongxi
He, Ming-Liang
author_facet Wang, Mengjie
Dong, Qi
Wang, Hua
He, Yaqing
Chen, Ying
Zhang, Hong
Wu, Rong
Chen, Xinchun
Zhou, Boping
He, Jason
Kung, Hsiang-Fu
Huang, Canhua
Wei, Yuquan
Huang, Jian-dong
Xu, Hongxi
He, Ming-Liang
author_sort Wang, Mengjie
collection PubMed
description There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.
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spelling pubmed-48910052016-06-20 Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57 Wang, Mengjie Dong, Qi Wang, Hua He, Yaqing Chen, Ying Zhang, Hong Wu, Rong Chen, Xinchun Zhou, Boping He, Jason Kung, Hsiang-Fu Huang, Canhua Wei, Yuquan Huang, Jian-dong Xu, Hongxi He, Ming-Liang Oncotarget Research Paper There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection. Impact Journals LLC 2016-02-01 /pmc/articles/PMC4891005/ /pubmed/26848777 http://dx.doi.org/10.18632/oncotarget.7122 Text en Copyright: © 2016 Wang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wang, Mengjie
Dong, Qi
Wang, Hua
He, Yaqing
Chen, Ying
Zhang, Hong
Wu, Rong
Chen, Xinchun
Zhou, Boping
He, Jason
Kung, Hsiang-Fu
Huang, Canhua
Wei, Yuquan
Huang, Jian-dong
Xu, Hongxi
He, Ming-Liang
Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
title Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
title_full Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
title_fullStr Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
title_full_unstemmed Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
title_short Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57
title_sort oblongifolin m, an active compound isolated from a chinese medical herb garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of erp57
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891005/
https://www.ncbi.nlm.nih.gov/pubmed/26848777
http://dx.doi.org/10.18632/oncotarget.7122
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