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High level of αB-crystallin contributes to the progression of osteosarcoma

Accumulating evidences indicate the elevated expression of αB-Crystallin (Cryab) is implicated in tumorigenesis. However, the expression and biologic role of Cryab in osteosarcoma (OS) are still unknown. In this study, we showed that Cryab expression was elevated in OS tissues and cell lines, and do...

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Autores principales: Shi, Qing-Ming, Luo, Jun, Wu, Kai, Yin, Ming, Gu, Yu-Rong, Cheng, Xi-Gao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891021/
https://www.ncbi.nlm.nih.gov/pubmed/26789112
http://dx.doi.org/10.18632/oncotarget.6928
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author Shi, Qing-Ming
Luo, Jun
Wu, Kai
Yin, Ming
Gu, Yu-Rong
Cheng, Xi-Gao
author_facet Shi, Qing-Ming
Luo, Jun
Wu, Kai
Yin, Ming
Gu, Yu-Rong
Cheng, Xi-Gao
author_sort Shi, Qing-Ming
collection PubMed
description Accumulating evidences indicate the elevated expression of αB-Crystallin (Cryab) is implicated in tumorigenesis. However, the expression and biologic role of Cryab in osteosarcoma (OS) are still unknown. In this study, we showed that Cryab expression was elevated in OS tissues and cell lines, and down-regulation of Cryab in MG-63 and U-2OS cells led to a decline in the cells’ aggressiveness, and reduced secretion of matrix metalloproteinase-9 (MMP-9) in vitro, and lower metastasis potential in vivo. Further study indicated that the Cryab expression was positively associated with the activity of ERK1/2 which is responsible for the cells’ aggressiveness and MMP-9 secretion. Clinically, our data confirmed that the high level of Cryab was associated with shorten survival and tumor recurrence for the postoperative OS patients. Together, our results indicate that high level of Cryab is a new adverse outcomes marker for OS patients and may be used as a new therapeutic target.
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spelling pubmed-48910212016-06-20 High level of αB-crystallin contributes to the progression of osteosarcoma Shi, Qing-Ming Luo, Jun Wu, Kai Yin, Ming Gu, Yu-Rong Cheng, Xi-Gao Oncotarget Research Paper Accumulating evidences indicate the elevated expression of αB-Crystallin (Cryab) is implicated in tumorigenesis. However, the expression and biologic role of Cryab in osteosarcoma (OS) are still unknown. In this study, we showed that Cryab expression was elevated in OS tissues and cell lines, and down-regulation of Cryab in MG-63 and U-2OS cells led to a decline in the cells’ aggressiveness, and reduced secretion of matrix metalloproteinase-9 (MMP-9) in vitro, and lower metastasis potential in vivo. Further study indicated that the Cryab expression was positively associated with the activity of ERK1/2 which is responsible for the cells’ aggressiveness and MMP-9 secretion. Clinically, our data confirmed that the high level of Cryab was associated with shorten survival and tumor recurrence for the postoperative OS patients. Together, our results indicate that high level of Cryab is a new adverse outcomes marker for OS patients and may be used as a new therapeutic target. Impact Journals LLC 2016-01-17 /pmc/articles/PMC4891021/ /pubmed/26789112 http://dx.doi.org/10.18632/oncotarget.6928 Text en Copyright: © 2016 Shi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Shi, Qing-Ming
Luo, Jun
Wu, Kai
Yin, Ming
Gu, Yu-Rong
Cheng, Xi-Gao
High level of αB-crystallin contributes to the progression of osteosarcoma
title High level of αB-crystallin contributes to the progression of osteosarcoma
title_full High level of αB-crystallin contributes to the progression of osteosarcoma
title_fullStr High level of αB-crystallin contributes to the progression of osteosarcoma
title_full_unstemmed High level of αB-crystallin contributes to the progression of osteosarcoma
title_short High level of αB-crystallin contributes to the progression of osteosarcoma
title_sort high level of αb-crystallin contributes to the progression of osteosarcoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891021/
https://www.ncbi.nlm.nih.gov/pubmed/26789112
http://dx.doi.org/10.18632/oncotarget.6928
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