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Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo
Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891044/ https://www.ncbi.nlm.nih.gov/pubmed/26824185 http://dx.doi.org/10.18632/oncotarget.7009 |
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author | Jiang, Zhengyu Zhang, Hongxia Wang, Ye Yu, Bin Wang, Chen Liu, Changcheng Lu, Juan Chen, Fei Wang, Minjun Yu, Xinlu Lin, Jiahao Pan, Xinghua Wang, Pin Zhu, Haiying |
author_facet | Jiang, Zhengyu Zhang, Hongxia Wang, Ye Yu, Bin Wang, Chen Liu, Changcheng Lu, Juan Chen, Fei Wang, Minjun Yu, Xinlu Lin, Jiahao Pan, Xinghua Wang, Pin Zhu, Haiying |
author_sort | Jiang, Zhengyu |
collection | PubMed |
description | Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D-hep cells, Hepa1-6 cells treated with DMSO for 7 days, could restore to the higher proliferation rate in DMSO-free medium, but alteration of gene expression profile was irreversible. Interestingly, tumors from D-hep cells, not Hepa1-6 cells, regressed in wild-type C57BL/6 mice whereas D-hep cells exhibited similar tumorigenesis as Hep1–6 cells in immunodeficient mice. As expected, additional Hepa1-6 cells failed to form tumors in the D-hep-C57 mice in which D-hep cells were eliminated. Further research confirmed that D-hep-C57 mice established anti-tumor immunity against Hepa1-6 cells. Our research proposed viable tumor cells with altered biological features by DMSO-treatment could induce anti-tumor immunity in vivo. |
format | Online Article Text |
id | pubmed-4891044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-48910442016-06-20 Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo Jiang, Zhengyu Zhang, Hongxia Wang, Ye Yu, Bin Wang, Chen Liu, Changcheng Lu, Juan Chen, Fei Wang, Minjun Yu, Xinlu Lin, Jiahao Pan, Xinghua Wang, Pin Zhu, Haiying Oncotarget Research Paper Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D-hep cells, Hepa1-6 cells treated with DMSO for 7 days, could restore to the higher proliferation rate in DMSO-free medium, but alteration of gene expression profile was irreversible. Interestingly, tumors from D-hep cells, not Hepa1-6 cells, regressed in wild-type C57BL/6 mice whereas D-hep cells exhibited similar tumorigenesis as Hep1–6 cells in immunodeficient mice. As expected, additional Hepa1-6 cells failed to form tumors in the D-hep-C57 mice in which D-hep cells were eliminated. Further research confirmed that D-hep-C57 mice established anti-tumor immunity against Hepa1-6 cells. Our research proposed viable tumor cells with altered biological features by DMSO-treatment could induce anti-tumor immunity in vivo. Impact Journals LLC 2016-01-25 /pmc/articles/PMC4891044/ /pubmed/26824185 http://dx.doi.org/10.18632/oncotarget.7009 Text en Copyright: © 2016 Jiang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Jiang, Zhengyu Zhang, Hongxia Wang, Ye Yu, Bin Wang, Chen Liu, Changcheng Lu, Juan Chen, Fei Wang, Minjun Yu, Xinlu Lin, Jiahao Pan, Xinghua Wang, Pin Zhu, Haiying Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo |
title | Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo |
title_full | Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo |
title_fullStr | Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo |
title_full_unstemmed | Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo |
title_short | Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo |
title_sort | altered hepa1-6 cells by dimethyl sulfoxide (dmso)-treatment induce anti-tumor immunity in vivo |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891044/ https://www.ncbi.nlm.nih.gov/pubmed/26824185 http://dx.doi.org/10.18632/oncotarget.7009 |
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