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MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue
Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to st...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891820/ https://www.ncbi.nlm.nih.gov/pubmed/27256770 http://dx.doi.org/10.1038/srep27106 |
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author | Mourino-Alvarez, Laura Iloro, Ibon de la Cuesta, Fernando Azkargorta, Mikel Sastre-Oliva, Tamara Escobes, Iraide Lopez-Almodovar, Luis F. Sanchez, Pedro L. Urreta, Harkaitz Fernandez-Aviles, Francisco Pinto, Angel Padial, Luis R. Akerström, Finn Elortza, Felix Barderas, Maria G. |
author_facet | Mourino-Alvarez, Laura Iloro, Ibon de la Cuesta, Fernando Azkargorta, Mikel Sastre-Oliva, Tamara Escobes, Iraide Lopez-Almodovar, Luis F. Sanchez, Pedro L. Urreta, Harkaitz Fernandez-Aviles, Francisco Pinto, Angel Padial, Luis R. Akerström, Finn Elortza, Felix Barderas, Maria G. |
author_sort | Mourino-Alvarez, Laura |
collection | PubMed |
description | Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to study the progression of calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) and get new insights at molecular level that could help in the understanding of this disease. In this work, we analyzed consecutive slices from aortic valve tissue by MALDI-IMS, to establish the spatial distribution of proteins and peptides directly from the surface of the histological sections. The analysis showed different structures corresponding to regions observed in conventional histology, including large calcification areas and zones rich in collagen and elastic fibers. Peptide extraction from the tissue, followed by liquid chromatography mass spectrometry analysis, provided the identification of collagen VI α-3 and NDRG2 proteins which correlated with the masses obtained by MALDI-IMS and were confirmed by immunohistochemistry. These results highlighted the molecular mechanism implied in AS using MALDI-IMS, a novel technique never used before in this pathology. In addition, we can define specific regions proving a complementary resolution of the molecular histology. |
format | Online Article Text |
id | pubmed-4891820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48918202016-06-10 MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue Mourino-Alvarez, Laura Iloro, Ibon de la Cuesta, Fernando Azkargorta, Mikel Sastre-Oliva, Tamara Escobes, Iraide Lopez-Almodovar, Luis F. Sanchez, Pedro L. Urreta, Harkaitz Fernandez-Aviles, Francisco Pinto, Angel Padial, Luis R. Akerström, Finn Elortza, Felix Barderas, Maria G. Sci Rep Article Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to study the progression of calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) and get new insights at molecular level that could help in the understanding of this disease. In this work, we analyzed consecutive slices from aortic valve tissue by MALDI-IMS, to establish the spatial distribution of proteins and peptides directly from the surface of the histological sections. The analysis showed different structures corresponding to regions observed in conventional histology, including large calcification areas and zones rich in collagen and elastic fibers. Peptide extraction from the tissue, followed by liquid chromatography mass spectrometry analysis, provided the identification of collagen VI α-3 and NDRG2 proteins which correlated with the masses obtained by MALDI-IMS and were confirmed by immunohistochemistry. These results highlighted the molecular mechanism implied in AS using MALDI-IMS, a novel technique never used before in this pathology. In addition, we can define specific regions proving a complementary resolution of the molecular histology. Nature Publishing Group 2016-06-03 /pmc/articles/PMC4891820/ /pubmed/27256770 http://dx.doi.org/10.1038/srep27106 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mourino-Alvarez, Laura Iloro, Ibon de la Cuesta, Fernando Azkargorta, Mikel Sastre-Oliva, Tamara Escobes, Iraide Lopez-Almodovar, Luis F. Sanchez, Pedro L. Urreta, Harkaitz Fernandez-Aviles, Francisco Pinto, Angel Padial, Luis R. Akerström, Finn Elortza, Felix Barderas, Maria G. MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
title | MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
title_full | MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
title_fullStr | MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
title_full_unstemmed | MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
title_short | MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
title_sort | maldi-imaging mass spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891820/ https://www.ncbi.nlm.nih.gov/pubmed/27256770 http://dx.doi.org/10.1038/srep27106 |
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