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Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar

Bacillus cereus isolates have been described harboring Bacillus anthracis toxin genes, most notably B. cereus G9241, and capable of causing severe and fatal pneumonias. This report describes the characterization of a B. cereus isolate, BcFL2013, associated with a naturally occurring cutaneous lesion...

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Autores principales: Marston, Chung K., Ibrahim, Hisham, Lee, Philip, Churchwell, George, Gumke, Megan, Stanek, Danielle, Gee, Jay E., Boyer, Anne E., Gallegos-Candela, Maribel, Barr, John R., Li, Han, Boulay, Darbi, Cronin, Li, Quinn, Conrad P., Hoffmaster, Alex R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892579/
https://www.ncbi.nlm.nih.gov/pubmed/27257909
http://dx.doi.org/10.1371/journal.pone.0156987
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author Marston, Chung K.
Ibrahim, Hisham
Lee, Philip
Churchwell, George
Gumke, Megan
Stanek, Danielle
Gee, Jay E.
Boyer, Anne E.
Gallegos-Candela, Maribel
Barr, John R.
Li, Han
Boulay, Darbi
Cronin, Li
Quinn, Conrad P.
Hoffmaster, Alex R.
author_facet Marston, Chung K.
Ibrahim, Hisham
Lee, Philip
Churchwell, George
Gumke, Megan
Stanek, Danielle
Gee, Jay E.
Boyer, Anne E.
Gallegos-Candela, Maribel
Barr, John R.
Li, Han
Boulay, Darbi
Cronin, Li
Quinn, Conrad P.
Hoffmaster, Alex R.
author_sort Marston, Chung K.
collection PubMed
description Bacillus cereus isolates have been described harboring Bacillus anthracis toxin genes, most notably B. cereus G9241, and capable of causing severe and fatal pneumonias. This report describes the characterization of a B. cereus isolate, BcFL2013, associated with a naturally occurring cutaneous lesion resembling an anthrax eschar. Similar to G9241, BcFL2013 is positive for the B. anthracis pXO1 toxin genes, has a multi-locus sequence type of 78, and a pagA sequence type of 9. Whole genome sequencing confirms the similarity to G9241. In addition to the chromosome having an average nucleotide identity of 99.98% when compared to G9241, BcFL2013 harbors three plasmids with varying homology to the G9241 plasmids (pBCXO1, pBC210 and pBFH_1). This is also the first report to include serologic testing of patient specimens associated with this type of B. cereus infection which resulted in the detection of anthrax lethal factor toxemia, a quantifiable serum antibody response to protective antigen (PA), and lethal toxin neutralization activity.
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spelling pubmed-48925792016-06-16 Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar Marston, Chung K. Ibrahim, Hisham Lee, Philip Churchwell, George Gumke, Megan Stanek, Danielle Gee, Jay E. Boyer, Anne E. Gallegos-Candela, Maribel Barr, John R. Li, Han Boulay, Darbi Cronin, Li Quinn, Conrad P. Hoffmaster, Alex R. PLoS One Research Article Bacillus cereus isolates have been described harboring Bacillus anthracis toxin genes, most notably B. cereus G9241, and capable of causing severe and fatal pneumonias. This report describes the characterization of a B. cereus isolate, BcFL2013, associated with a naturally occurring cutaneous lesion resembling an anthrax eschar. Similar to G9241, BcFL2013 is positive for the B. anthracis pXO1 toxin genes, has a multi-locus sequence type of 78, and a pagA sequence type of 9. Whole genome sequencing confirms the similarity to G9241. In addition to the chromosome having an average nucleotide identity of 99.98% when compared to G9241, BcFL2013 harbors three plasmids with varying homology to the G9241 plasmids (pBCXO1, pBC210 and pBFH_1). This is also the first report to include serologic testing of patient specimens associated with this type of B. cereus infection which resulted in the detection of anthrax lethal factor toxemia, a quantifiable serum antibody response to protective antigen (PA), and lethal toxin neutralization activity. Public Library of Science 2016-06-03 /pmc/articles/PMC4892579/ /pubmed/27257909 http://dx.doi.org/10.1371/journal.pone.0156987 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Marston, Chung K.
Ibrahim, Hisham
Lee, Philip
Churchwell, George
Gumke, Megan
Stanek, Danielle
Gee, Jay E.
Boyer, Anne E.
Gallegos-Candela, Maribel
Barr, John R.
Li, Han
Boulay, Darbi
Cronin, Li
Quinn, Conrad P.
Hoffmaster, Alex R.
Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar
title Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar
title_full Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar
title_fullStr Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar
title_full_unstemmed Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar
title_short Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar
title_sort anthrax toxin-expressing bacillus cereus isolated from an anthrax-like eschar
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892579/
https://www.ncbi.nlm.nih.gov/pubmed/27257909
http://dx.doi.org/10.1371/journal.pone.0156987
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