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Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid

Restricted usage of chemical nematicides has led to development of environmentally safe alternatives. A culture filtrate of Aspergillus niger F22 was highly active against Meloidogyne incognita with marked mortality of second-stage juveniles (J2s) and inhibition of egg hatching. The nematicidal comp...

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Autores principales: Jang, Ja Yeong, Choi, Yong Ho, Shin, Teak Soo, Kim, Tae Hoon, Shin, Kee-Sun, Park, Hae Woong, Kim, Young Ho, Kim, Hun, Choi, Gyung Ja, Jang, Kyoung Soo, Cha, Byeongjin, Kim, In Seon, Myung, Eul Jae, Kim, Jin-Cheol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892604/
https://www.ncbi.nlm.nih.gov/pubmed/27258452
http://dx.doi.org/10.1371/journal.pone.0156230
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author Jang, Ja Yeong
Choi, Yong Ho
Shin, Teak Soo
Kim, Tae Hoon
Shin, Kee-Sun
Park, Hae Woong
Kim, Young Ho
Kim, Hun
Choi, Gyung Ja
Jang, Kyoung Soo
Cha, Byeongjin
Kim, In Seon
Myung, Eul Jae
Kim, Jin-Cheol
author_facet Jang, Ja Yeong
Choi, Yong Ho
Shin, Teak Soo
Kim, Tae Hoon
Shin, Kee-Sun
Park, Hae Woong
Kim, Young Ho
Kim, Hun
Choi, Gyung Ja
Jang, Kyoung Soo
Cha, Byeongjin
Kim, In Seon
Myung, Eul Jae
Kim, Jin-Cheol
author_sort Jang, Ja Yeong
collection PubMed
description Restricted usage of chemical nematicides has led to development of environmentally safe alternatives. A culture filtrate of Aspergillus niger F22 was highly active against Meloidogyne incognita with marked mortality of second-stage juveniles (J2s) and inhibition of egg hatching. The nematicidal component was identified as oxalic acid by organic acid analysis and gas chromatography-mass spectroscopy (GC-MS). Exposure to 2 mmol/L oxalic acid resulted in 100% juvenile mortality at 1 day after treatment and suppressed egg hatching by 95.6% at 7 days after treatment. Oxalic acid showed similar nematicidal activity against M. hapla, but was not highly toxic to Bursaphelenchus xylophilus. The fungus was incubated on solid medium and dried culture was used for preparation of a wettable powder-type (WP) formulation as an active ingredient. Two WP formulations, F22-WP10 (ai 10%) and oxalic acid-WP8 (ai 8%), were prepared using F22 solid culture and oxalic acid. In a field naturally infested with M. incognita, application of a mixture of F22-WP10 + oxalic acid-WP8 at 1,000- and 500-fold dilutions significantly reduced gall formation on the roots of watermelon plants by 58.8 and 70.7%, respectively, compared to the non-treated control. The disease control efficacy of the mixture of F22-WP10 + oxalic acid-WP8 was significantly higher than that of a chemical nematicide, Sunchungtan (ai 30% fosthiazate). These results suggest that A. niger F22 can be used as a microbial nematicide for the control of root-knot nematode disease.
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spelling pubmed-48926042016-06-16 Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid Jang, Ja Yeong Choi, Yong Ho Shin, Teak Soo Kim, Tae Hoon Shin, Kee-Sun Park, Hae Woong Kim, Young Ho Kim, Hun Choi, Gyung Ja Jang, Kyoung Soo Cha, Byeongjin Kim, In Seon Myung, Eul Jae Kim, Jin-Cheol PLoS One Research Article Restricted usage of chemical nematicides has led to development of environmentally safe alternatives. A culture filtrate of Aspergillus niger F22 was highly active against Meloidogyne incognita with marked mortality of second-stage juveniles (J2s) and inhibition of egg hatching. The nematicidal component was identified as oxalic acid by organic acid analysis and gas chromatography-mass spectroscopy (GC-MS). Exposure to 2 mmol/L oxalic acid resulted in 100% juvenile mortality at 1 day after treatment and suppressed egg hatching by 95.6% at 7 days after treatment. Oxalic acid showed similar nematicidal activity against M. hapla, but was not highly toxic to Bursaphelenchus xylophilus. The fungus was incubated on solid medium and dried culture was used for preparation of a wettable powder-type (WP) formulation as an active ingredient. Two WP formulations, F22-WP10 (ai 10%) and oxalic acid-WP8 (ai 8%), were prepared using F22 solid culture and oxalic acid. In a field naturally infested with M. incognita, application of a mixture of F22-WP10 + oxalic acid-WP8 at 1,000- and 500-fold dilutions significantly reduced gall formation on the roots of watermelon plants by 58.8 and 70.7%, respectively, compared to the non-treated control. The disease control efficacy of the mixture of F22-WP10 + oxalic acid-WP8 was significantly higher than that of a chemical nematicide, Sunchungtan (ai 30% fosthiazate). These results suggest that A. niger F22 can be used as a microbial nematicide for the control of root-knot nematode disease. Public Library of Science 2016-06-03 /pmc/articles/PMC4892604/ /pubmed/27258452 http://dx.doi.org/10.1371/journal.pone.0156230 Text en © 2016 Jang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jang, Ja Yeong
Choi, Yong Ho
Shin, Teak Soo
Kim, Tae Hoon
Shin, Kee-Sun
Park, Hae Woong
Kim, Young Ho
Kim, Hun
Choi, Gyung Ja
Jang, Kyoung Soo
Cha, Byeongjin
Kim, In Seon
Myung, Eul Jae
Kim, Jin-Cheol
Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid
title Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid
title_full Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid
title_fullStr Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid
title_full_unstemmed Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid
title_short Biological Control of Meloidogyne incognita by Aspergillus niger F22 Producing Oxalic Acid
title_sort biological control of meloidogyne incognita by aspergillus niger f22 producing oxalic acid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892604/
https://www.ncbi.nlm.nih.gov/pubmed/27258452
http://dx.doi.org/10.1371/journal.pone.0156230
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