Cargando…

A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing

Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely u...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Daoyang, Nandety, Raja Sekhar, Zhang, Yanlong, Reid, Michael S., Niu, Lixin, Jiang, Cai-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892726/
https://www.ncbi.nlm.nih.gov/pubmed/27099376
http://dx.doi.org/10.1093/jxb/erw155
_version_ 1782435444779122688
author Sun, Daoyang
Nandety, Raja Sekhar
Zhang, Yanlong
Reid, Michael S.
Niu, Lixin
Jiang, Cai-Zhong
author_facet Sun, Daoyang
Nandety, Raja Sekhar
Zhang, Yanlong
Reid, Michael S.
Niu, Lixin
Jiang, Cai-Zhong
author_sort Sun, Daoyang
collection PubMed
description Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, is induced by Tobacco rattle virus (TRV) infection. Inclusion of a PhERF2 fragment in a TRV silencing construct containing reporter fragments of phytoene desaturase (PDS) or chalcone synthase (CHS) substantially impaired silencing efficiency of both the PDS and CHS reporters. Silencing was also impaired in PhERF2- RNAi lines, where TRV-PhPDS infection did not show the expected silencing phenotype (photobleaching). In contrast, photobleaching in response to infiltration with the TRV-PhPDS construct was enhanced in plants overexpressing PhERF2. Transcript abundance of the RNA silencing-related genes RDR2, RDR6, DCL2, and AGO2 was lower in PhERF2-silenced plants but higher in PhERF2-overexpressing plants. Moreover, PhERF2-silenced lines showed higher susceptibility to Cucumber mosaic virus (CMV) than wild-type (WT) plants, while plants overexpressing PhERF2 exhibited increased resistance. Interestingly, growth and development of PhERF2-RNAi lines were substantially slower, whereas the overexpressing lines were more vigorous than the controls. Taken together, our results indicate that PhERF2 functions as a positive regulator in antiviral RNA silencing.
format Online
Article
Text
id pubmed-4892726
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-48927262016-06-07 A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing Sun, Daoyang Nandety, Raja Sekhar Zhang, Yanlong Reid, Michael S. Niu, Lixin Jiang, Cai-Zhong J Exp Bot Research Paper Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, is induced by Tobacco rattle virus (TRV) infection. Inclusion of a PhERF2 fragment in a TRV silencing construct containing reporter fragments of phytoene desaturase (PDS) or chalcone synthase (CHS) substantially impaired silencing efficiency of both the PDS and CHS reporters. Silencing was also impaired in PhERF2- RNAi lines, where TRV-PhPDS infection did not show the expected silencing phenotype (photobleaching). In contrast, photobleaching in response to infiltration with the TRV-PhPDS construct was enhanced in plants overexpressing PhERF2. Transcript abundance of the RNA silencing-related genes RDR2, RDR6, DCL2, and AGO2 was lower in PhERF2-silenced plants but higher in PhERF2-overexpressing plants. Moreover, PhERF2-silenced lines showed higher susceptibility to Cucumber mosaic virus (CMV) than wild-type (WT) plants, while plants overexpressing PhERF2 exhibited increased resistance. Interestingly, growth and development of PhERF2-RNAi lines were substantially slower, whereas the overexpressing lines were more vigorous than the controls. Taken together, our results indicate that PhERF2 functions as a positive regulator in antiviral RNA silencing. Oxford University Press 2016-05 2016-04-19 /pmc/articles/PMC4892726/ /pubmed/27099376 http://dx.doi.org/10.1093/jxb/erw155 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Sun, Daoyang
Nandety, Raja Sekhar
Zhang, Yanlong
Reid, Michael S.
Niu, Lixin
Jiang, Cai-Zhong
A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
title A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
title_full A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
title_fullStr A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
title_full_unstemmed A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
title_short A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
title_sort petunia ethylene-responsive element binding factor, pherf2, plays an important role in antiviral rna silencing
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892726/
https://www.ncbi.nlm.nih.gov/pubmed/27099376
http://dx.doi.org/10.1093/jxb/erw155
work_keys_str_mv AT sundaoyang apetuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT nandetyrajasekhar apetuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT zhangyanlong apetuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT reidmichaels apetuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT niulixin apetuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT jiangcaizhong apetuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT sundaoyang petuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT nandetyrajasekhar petuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT zhangyanlong petuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT reidmichaels petuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT niulixin petuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing
AT jiangcaizhong petuniaethyleneresponsiveelementbindingfactorpherf2playsanimportantroleinantiviralrnasilencing