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A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing
Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely u...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892726/ https://www.ncbi.nlm.nih.gov/pubmed/27099376 http://dx.doi.org/10.1093/jxb/erw155 |
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author | Sun, Daoyang Nandety, Raja Sekhar Zhang, Yanlong Reid, Michael S. Niu, Lixin Jiang, Cai-Zhong |
author_facet | Sun, Daoyang Nandety, Raja Sekhar Zhang, Yanlong Reid, Michael S. Niu, Lixin Jiang, Cai-Zhong |
author_sort | Sun, Daoyang |
collection | PubMed |
description | Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, is induced by Tobacco rattle virus (TRV) infection. Inclusion of a PhERF2 fragment in a TRV silencing construct containing reporter fragments of phytoene desaturase (PDS) or chalcone synthase (CHS) substantially impaired silencing efficiency of both the PDS and CHS reporters. Silencing was also impaired in PhERF2- RNAi lines, where TRV-PhPDS infection did not show the expected silencing phenotype (photobleaching). In contrast, photobleaching in response to infiltration with the TRV-PhPDS construct was enhanced in plants overexpressing PhERF2. Transcript abundance of the RNA silencing-related genes RDR2, RDR6, DCL2, and AGO2 was lower in PhERF2-silenced plants but higher in PhERF2-overexpressing plants. Moreover, PhERF2-silenced lines showed higher susceptibility to Cucumber mosaic virus (CMV) than wild-type (WT) plants, while plants overexpressing PhERF2 exhibited increased resistance. Interestingly, growth and development of PhERF2-RNAi lines were substantially slower, whereas the overexpressing lines were more vigorous than the controls. Taken together, our results indicate that PhERF2 functions as a positive regulator in antiviral RNA silencing. |
format | Online Article Text |
id | pubmed-4892726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48927262016-06-07 A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing Sun, Daoyang Nandety, Raja Sekhar Zhang, Yanlong Reid, Michael S. Niu, Lixin Jiang, Cai-Zhong J Exp Bot Research Paper Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, is induced by Tobacco rattle virus (TRV) infection. Inclusion of a PhERF2 fragment in a TRV silencing construct containing reporter fragments of phytoene desaturase (PDS) or chalcone synthase (CHS) substantially impaired silencing efficiency of both the PDS and CHS reporters. Silencing was also impaired in PhERF2- RNAi lines, where TRV-PhPDS infection did not show the expected silencing phenotype (photobleaching). In contrast, photobleaching in response to infiltration with the TRV-PhPDS construct was enhanced in plants overexpressing PhERF2. Transcript abundance of the RNA silencing-related genes RDR2, RDR6, DCL2, and AGO2 was lower in PhERF2-silenced plants but higher in PhERF2-overexpressing plants. Moreover, PhERF2-silenced lines showed higher susceptibility to Cucumber mosaic virus (CMV) than wild-type (WT) plants, while plants overexpressing PhERF2 exhibited increased resistance. Interestingly, growth and development of PhERF2-RNAi lines were substantially slower, whereas the overexpressing lines were more vigorous than the controls. Taken together, our results indicate that PhERF2 functions as a positive regulator in antiviral RNA silencing. Oxford University Press 2016-05 2016-04-19 /pmc/articles/PMC4892726/ /pubmed/27099376 http://dx.doi.org/10.1093/jxb/erw155 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Sun, Daoyang Nandety, Raja Sekhar Zhang, Yanlong Reid, Michael S. Niu, Lixin Jiang, Cai-Zhong A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing |
title | A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing |
title_full | A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing |
title_fullStr | A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing |
title_full_unstemmed | A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing |
title_short | A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing |
title_sort | petunia ethylene-responsive element binding factor, pherf2, plays an important role in antiviral rna silencing |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892726/ https://www.ncbi.nlm.nih.gov/pubmed/27099376 http://dx.doi.org/10.1093/jxb/erw155 |
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