Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes

Mitochondrial cytochrome c oxidase utilizes electrons provided by cytochrome c for the active vectorial transport of protons across the inner mitochondrial membrane through the reduction of molecular oxygen to water. Direct structural evidence on the transient cytochrome c oxidase–cytochrome c compl...

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Detalles Bibliográficos
Autores principales: Osuda, Yukiho, Shinzawa-Itoh, Kyoko, Tani, Kazutoshi, Maeda, Shintaro, Yoshikawa, Shinya, Tsukihara, Tomitake, Gerle, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892887/
https://www.ncbi.nlm.nih.gov/pubmed/26754561
http://dx.doi.org/10.1093/jmicro/dfv381
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author Osuda, Yukiho
Shinzawa-Itoh, Kyoko
Tani, Kazutoshi
Maeda, Shintaro
Yoshikawa, Shinya
Tsukihara, Tomitake
Gerle, Christoph
author_facet Osuda, Yukiho
Shinzawa-Itoh, Kyoko
Tani, Kazutoshi
Maeda, Shintaro
Yoshikawa, Shinya
Tsukihara, Tomitake
Gerle, Christoph
author_sort Osuda, Yukiho
collection PubMed
description Mitochondrial cytochrome c oxidase utilizes electrons provided by cytochrome c for the active vectorial transport of protons across the inner mitochondrial membrane through the reduction of molecular oxygen to water. Direct structural evidence on the transient cytochrome c oxidase–cytochrome c complex thus far, however, remains elusive and its physiological relevant oligomeric form is unclear. Here, we report on the 2D crystallization of monomeric bovine cytochrome c oxidase with tightly bound cytochrome c at a molar ratio of 1:1 in reconstituted lipid membranes at the basic pH of 8.5 and low ionic strength.
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spelling pubmed-48928872016-06-07 Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes Osuda, Yukiho Shinzawa-Itoh, Kyoko Tani, Kazutoshi Maeda, Shintaro Yoshikawa, Shinya Tsukihara, Tomitake Gerle, Christoph Microscopy (Oxf) Letter Mitochondrial cytochrome c oxidase utilizes electrons provided by cytochrome c for the active vectorial transport of protons across the inner mitochondrial membrane through the reduction of molecular oxygen to water. Direct structural evidence on the transient cytochrome c oxidase–cytochrome c complex thus far, however, remains elusive and its physiological relevant oligomeric form is unclear. Here, we report on the 2D crystallization of monomeric bovine cytochrome c oxidase with tightly bound cytochrome c at a molar ratio of 1:1 in reconstituted lipid membranes at the basic pH of 8.5 and low ionic strength. Oxford University Press 2016-06 2016-01-10 /pmc/articles/PMC4892887/ /pubmed/26754561 http://dx.doi.org/10.1093/jmicro/dfv381 Text en © The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited
spellingShingle Letter
Osuda, Yukiho
Shinzawa-Itoh, Kyoko
Tani, Kazutoshi
Maeda, Shintaro
Yoshikawa, Shinya
Tsukihara, Tomitake
Gerle, Christoph
Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
title Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
title_full Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
title_fullStr Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
title_full_unstemmed Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
title_short Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
title_sort two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892887/
https://www.ncbi.nlm.nih.gov/pubmed/26754561
http://dx.doi.org/10.1093/jmicro/dfv381
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