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Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy
BACKGROUND: Leiomodin proteins, Lmod1, Lmod2 and Lmod3, are key regulators of the thin filament length in muscles. While Lmod1 is specifically expressed in smooth muscles, both Lmod2 and Lmod3 are expressed in striated muscles including both cardiac and skeletal muscles. We and others have previousl...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893230/ https://www.ncbi.nlm.nih.gov/pubmed/27274810 http://dx.doi.org/10.1186/s13578-016-0101-y |
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author | Li, Shuang Mo, Kaiqi Tian, Hong Chu, Chen Sun, Shuna Tian, Lei Ding, Sheng Li, Tong-ruei Wu, Xiaohui Liu, Fang Zhang, Zhen Xu, Tian Sun, Ling V. |
author_facet | Li, Shuang Mo, Kaiqi Tian, Hong Chu, Chen Sun, Shuna Tian, Lei Ding, Sheng Li, Tong-ruei Wu, Xiaohui Liu, Fang Zhang, Zhen Xu, Tian Sun, Ling V. |
author_sort | Li, Shuang |
collection | PubMed |
description | BACKGROUND: Leiomodin proteins, Lmod1, Lmod2 and Lmod3, are key regulators of the thin filament length in muscles. While Lmod1 is specifically expressed in smooth muscles, both Lmod2 and Lmod3 are expressed in striated muscles including both cardiac and skeletal muscles. We and others have previously shown that Lmod3 mainly function in skeletal muscles and the mutant mice display disorganized sarcomere. Lmod2 protein has been found to act as an actin filament nucleator in both cell-free assays and in cultured rat and chicken cardiomyocytes. RESULTS: To better understand the function of Lmod2 in vivo, we have identified and characterized a piggyBac (PB) insertional mouse mutant. Our analysis revealed that the PB transposon inserts in the first exon of the Lmod2 gene and severely disrupts its expression. We found that Lmod2(PB/PB) mice exhibit typical dilated cardiomyopathy (DCM) with ventricular arrhythmias and postnatal lethality. Electron microscope reveals that the Lmod2(PB/PB) hearts carry disordered sarcomere, disarrayed thin filaments, and distorted intercalated discs (ICDs). Those ICDs display not only decreased convolutions, but also reduced electron-dense staining, indicating less ICDs component proteins in Lmod2(PB/PB) hearts. Consistent with the phenotype, the expression of the ICD component genes, β-catenin and Connexin43, are down-regulated. CONCLUSIONS: Taken together, our data reveal that Lmod2 is required in heart thin filaments for integrity of sarcomere and ICD and deficient mice exhibit DCM with ventricular arrhythmias and postnatal lethality. The Lmod2(PB/PB) mutant offers a valuable resource for interrogation of pathogenesis and development of therapeutics for DCM. |
format | Online Article Text |
id | pubmed-4893230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48932302016-06-05 Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy Li, Shuang Mo, Kaiqi Tian, Hong Chu, Chen Sun, Shuna Tian, Lei Ding, Sheng Li, Tong-ruei Wu, Xiaohui Liu, Fang Zhang, Zhen Xu, Tian Sun, Ling V. Cell Biosci Research BACKGROUND: Leiomodin proteins, Lmod1, Lmod2 and Lmod3, are key regulators of the thin filament length in muscles. While Lmod1 is specifically expressed in smooth muscles, both Lmod2 and Lmod3 are expressed in striated muscles including both cardiac and skeletal muscles. We and others have previously shown that Lmod3 mainly function in skeletal muscles and the mutant mice display disorganized sarcomere. Lmod2 protein has been found to act as an actin filament nucleator in both cell-free assays and in cultured rat and chicken cardiomyocytes. RESULTS: To better understand the function of Lmod2 in vivo, we have identified and characterized a piggyBac (PB) insertional mouse mutant. Our analysis revealed that the PB transposon inserts in the first exon of the Lmod2 gene and severely disrupts its expression. We found that Lmod2(PB/PB) mice exhibit typical dilated cardiomyopathy (DCM) with ventricular arrhythmias and postnatal lethality. Electron microscope reveals that the Lmod2(PB/PB) hearts carry disordered sarcomere, disarrayed thin filaments, and distorted intercalated discs (ICDs). Those ICDs display not only decreased convolutions, but also reduced electron-dense staining, indicating less ICDs component proteins in Lmod2(PB/PB) hearts. Consistent with the phenotype, the expression of the ICD component genes, β-catenin and Connexin43, are down-regulated. CONCLUSIONS: Taken together, our data reveal that Lmod2 is required in heart thin filaments for integrity of sarcomere and ICD and deficient mice exhibit DCM with ventricular arrhythmias and postnatal lethality. The Lmod2(PB/PB) mutant offers a valuable resource for interrogation of pathogenesis and development of therapeutics for DCM. BioMed Central 2016-06-04 /pmc/articles/PMC4893230/ /pubmed/27274810 http://dx.doi.org/10.1186/s13578-016-0101-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Shuang Mo, Kaiqi Tian, Hong Chu, Chen Sun, Shuna Tian, Lei Ding, Sheng Li, Tong-ruei Wu, Xiaohui Liu, Fang Zhang, Zhen Xu, Tian Sun, Ling V. Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy |
title | Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy |
title_full | Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy |
title_fullStr | Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy |
title_full_unstemmed | Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy |
title_short | Lmod2piggyBac mutant mice exhibit dilated cardiomyopathy |
title_sort | lmod2piggybac mutant mice exhibit dilated cardiomyopathy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893230/ https://www.ncbi.nlm.nih.gov/pubmed/27274810 http://dx.doi.org/10.1186/s13578-016-0101-y |
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