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MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma
Hypoxia is a common feature in solid tumours. In glioma, it is considered the major driving force for tumour angiogenesis and correlates with enhanced resistance to conventional therapies, increased invasiveness and a poor prognosis for patients. Here we describe, for the first time, that miR675-5p,...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893639/ https://www.ncbi.nlm.nih.gov/pubmed/27279905 http://dx.doi.org/10.7150/thno.14700 |
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author | Lo Dico, Alessia Costa, Viviana Martelli, Cristina Diceglie, Cecilia Rajata, Francesca Rizzo, Aroldo Mancone, Carmine Tripodi, Marco Ottobrini, Luisa Alessandro, Riccardo Conigliaro, Alice |
author_facet | Lo Dico, Alessia Costa, Viviana Martelli, Cristina Diceglie, Cecilia Rajata, Francesca Rizzo, Aroldo Mancone, Carmine Tripodi, Marco Ottobrini, Luisa Alessandro, Riccardo Conigliaro, Alice |
author_sort | Lo Dico, Alessia |
collection | PubMed |
description | Hypoxia is a common feature in solid tumours. In glioma, it is considered the major driving force for tumour angiogenesis and correlates with enhanced resistance to conventional therapies, increased invasiveness and a poor prognosis for patients. Here we describe, for the first time, that miR675-5p, embedded in hypoxia-induced long non-coding RNA H19, plays a mandatory role in establishing a hypoxic response and in promoting hypoxia-mediated angiogenesis. We demonstrated, in vitro and in vivo, that miR675-5p over expression in normoxia is sufficient to induce a hypoxic moreover, miR675-5p depletion in low oxygen conditions, drastically abolishes hypoxic responses including angiogenesis. In addition, our data indicate an interaction of miR675-5p, HIF-1α mRNA and the RNA Binding Protein HuR in hypoxia-induced responses. We suggest the modulation of miR675-5p as a new therapeutic option to promote or abolish hypoxia induced angiogenesis. |
format | Online Article Text |
id | pubmed-4893639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-48936392016-06-08 MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma Lo Dico, Alessia Costa, Viviana Martelli, Cristina Diceglie, Cecilia Rajata, Francesca Rizzo, Aroldo Mancone, Carmine Tripodi, Marco Ottobrini, Luisa Alessandro, Riccardo Conigliaro, Alice Theranostics Research Paper Hypoxia is a common feature in solid tumours. In glioma, it is considered the major driving force for tumour angiogenesis and correlates with enhanced resistance to conventional therapies, increased invasiveness and a poor prognosis for patients. Here we describe, for the first time, that miR675-5p, embedded in hypoxia-induced long non-coding RNA H19, plays a mandatory role in establishing a hypoxic response and in promoting hypoxia-mediated angiogenesis. We demonstrated, in vitro and in vivo, that miR675-5p over expression in normoxia is sufficient to induce a hypoxic moreover, miR675-5p depletion in low oxygen conditions, drastically abolishes hypoxic responses including angiogenesis. In addition, our data indicate an interaction of miR675-5p, HIF-1α mRNA and the RNA Binding Protein HuR in hypoxia-induced responses. We suggest the modulation of miR675-5p as a new therapeutic option to promote or abolish hypoxia induced angiogenesis. Ivyspring International Publisher 2016-05-08 /pmc/articles/PMC4893639/ /pubmed/27279905 http://dx.doi.org/10.7150/thno.14700 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Lo Dico, Alessia Costa, Viviana Martelli, Cristina Diceglie, Cecilia Rajata, Francesca Rizzo, Aroldo Mancone, Carmine Tripodi, Marco Ottobrini, Luisa Alessandro, Riccardo Conigliaro, Alice MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma |
title | MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma |
title_full | MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma |
title_fullStr | MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma |
title_full_unstemmed | MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma |
title_short | MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma |
title_sort | mir675-5p acts on hif-1α to sustain hypoxic responses: a new therapeutic strategy for glioma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893639/ https://www.ncbi.nlm.nih.gov/pubmed/27279905 http://dx.doi.org/10.7150/thno.14700 |
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