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Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride

Carcinoembryonic antigen (CEA) was used as the model, an ultrasensitive label-free photoelectrochemical immunosensor was developed using 2D TiO(2) nanosheets and carboxylated graphitic carbon nitride (g-C(3)N(4)) as photoactive materials and ascorbic acid as an efficient electron donor. 2D TiO(2) na...

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Autores principales: Wang, Huan, Wang, Yaoguang, Zhang, Yong, Wang, Qi, Ren, Xiang, Wu, Dan, Wei, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893710/
https://www.ncbi.nlm.nih.gov/pubmed/27263659
http://dx.doi.org/10.1038/srep27385
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author Wang, Huan
Wang, Yaoguang
Zhang, Yong
Wang, Qi
Ren, Xiang
Wu, Dan
Wei, Qin
author_facet Wang, Huan
Wang, Yaoguang
Zhang, Yong
Wang, Qi
Ren, Xiang
Wu, Dan
Wei, Qin
author_sort Wang, Huan
collection PubMed
description Carcinoembryonic antigen (CEA) was used as the model, an ultrasensitive label-free photoelectrochemical immunosensor was developed using 2D TiO(2) nanosheets and carboxylated graphitic carbon nitride (g-C(3)N(4)) as photoactive materials and ascorbic acid as an efficient electron donor. 2D TiO(2) nanosheets was sythsized by surfactant self-assembly method and proved to have higher photoelectrochemical signals than TiO(2) nanoparticles. Firstly, carboxylated g-C(3)N(4) could be attached to 2D TiO(2) nanosheets through the bond formed between carboxyl group of carboxylated g-C(3)N(4) and TiO(2). And the photocurrent of g-C(3)N(4)/TiO(2) drastically enhances compared to carboxylated g-C(3)N(4) and TiO(2). Then, antibody of CEA was bonded to TiO(2) through the dentate bond formed between carboxyl group of anti-CEA and TiO(2), leading to the decrease of the photocurrents. As proven by PEC experiments and electrochemical impedance spectroscopy (EIS) analysis, the fabrication process of the immunosensor is successful. Under the optimal conditions, the intensity decreased linearly with CEA concentration in the range of 0.01~10 ng/mL. The detection limit is 2.1 pg/mL. The work provides an effective method for the detection of tumor markers and can be extended for the application in food safety and environmental monitoring analysis.
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spelling pubmed-48937102016-06-10 Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride Wang, Huan Wang, Yaoguang Zhang, Yong Wang, Qi Ren, Xiang Wu, Dan Wei, Qin Sci Rep Article Carcinoembryonic antigen (CEA) was used as the model, an ultrasensitive label-free photoelectrochemical immunosensor was developed using 2D TiO(2) nanosheets and carboxylated graphitic carbon nitride (g-C(3)N(4)) as photoactive materials and ascorbic acid as an efficient electron donor. 2D TiO(2) nanosheets was sythsized by surfactant self-assembly method and proved to have higher photoelectrochemical signals than TiO(2) nanoparticles. Firstly, carboxylated g-C(3)N(4) could be attached to 2D TiO(2) nanosheets through the bond formed between carboxyl group of carboxylated g-C(3)N(4) and TiO(2). And the photocurrent of g-C(3)N(4)/TiO(2) drastically enhances compared to carboxylated g-C(3)N(4) and TiO(2). Then, antibody of CEA was bonded to TiO(2) through the dentate bond formed between carboxyl group of anti-CEA and TiO(2), leading to the decrease of the photocurrents. As proven by PEC experiments and electrochemical impedance spectroscopy (EIS) analysis, the fabrication process of the immunosensor is successful. Under the optimal conditions, the intensity decreased linearly with CEA concentration in the range of 0.01~10 ng/mL. The detection limit is 2.1 pg/mL. The work provides an effective method for the detection of tumor markers and can be extended for the application in food safety and environmental monitoring analysis. Nature Publishing Group 2016-06-06 /pmc/articles/PMC4893710/ /pubmed/27263659 http://dx.doi.org/10.1038/srep27385 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Huan
Wang, Yaoguang
Zhang, Yong
Wang, Qi
Ren, Xiang
Wu, Dan
Wei, Qin
Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride
title Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride
title_full Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride
title_fullStr Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride
title_full_unstemmed Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride
title_short Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO(2) Nanosheets and Carboxylated Graphitic Carbon Nitride
title_sort photoelectrochemical immunosensor for detection of carcinoembryonic antigen based on 2d tio(2) nanosheets and carboxylated graphitic carbon nitride
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893710/
https://www.ncbi.nlm.nih.gov/pubmed/27263659
http://dx.doi.org/10.1038/srep27385
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