Cargando…

Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria

AIM: To determine the presence of MRSA in hunting dogs in Maiduguri metropolitan. MATERIALS AND METHODS: Phenotypic methods used includes microscopic technique, colony morphology study, catalase-coagulase tests, and the use of mannitol salt agar test, oxacillin resistance screening agar base, and an...

Descripción completa

Detalles Bibliográficos
Autores principales: Mustapha, Muhammad, Bukar-Kolo, Yachilla Maryam, Geidam, Yaqub Ahmed, Gulani, Isa Adamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893722/
https://www.ncbi.nlm.nih.gov/pubmed/27284227
http://dx.doi.org/10.14202/vetworld.2016.501-506
_version_ 1782435612184281088
author Mustapha, Muhammad
Bukar-Kolo, Yachilla Maryam
Geidam, Yaqub Ahmed
Gulani, Isa Adamu
author_facet Mustapha, Muhammad
Bukar-Kolo, Yachilla Maryam
Geidam, Yaqub Ahmed
Gulani, Isa Adamu
author_sort Mustapha, Muhammad
collection PubMed
description AIM: To determine the presence of MRSA in hunting dogs in Maiduguri metropolitan. MATERIALS AND METHODS: Phenotypic methods used includes microscopic technique, colony morphology study, catalase-coagulase tests, and the use of mannitol salt agar test, oxacillin resistance screening agar base, and antibiotic susceptibility testing methods. Genotypic approach was used for deoxyribonucleic acid extraction, and the presence of nuc and mecA gene was detected using polymerase chain reaction (PCR) techniques. RESULTS: Examination of 416 swab samples from nasal and perineal region of dogs revealed a total of 79.5% of S. aureus, where 62.5% of the isolates were MRSA. Molecular analysis revealed that 7nuc genes specific for S. aureus from 20 presumptive MRSA assay were all mecA PCR negative. The isolates were sensitive to gentamicin and ciprofloxacin but proved resistant to cefoxitin and oxacillin. CONCLUSION: High isolation rate of MRSA was found in hunting dogs. Significant level (p<0.05) of MRSA was isolated in the nasal cavity of hunting dogs than its perineum. Only nuc genes were detected from the MRSA isolates.
format Online
Article
Text
id pubmed-4893722
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-48937222016-06-09 Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria Mustapha, Muhammad Bukar-Kolo, Yachilla Maryam Geidam, Yaqub Ahmed Gulani, Isa Adamu Vet World Research Article AIM: To determine the presence of MRSA in hunting dogs in Maiduguri metropolitan. MATERIALS AND METHODS: Phenotypic methods used includes microscopic technique, colony morphology study, catalase-coagulase tests, and the use of mannitol salt agar test, oxacillin resistance screening agar base, and antibiotic susceptibility testing methods. Genotypic approach was used for deoxyribonucleic acid extraction, and the presence of nuc and mecA gene was detected using polymerase chain reaction (PCR) techniques. RESULTS: Examination of 416 swab samples from nasal and perineal region of dogs revealed a total of 79.5% of S. aureus, where 62.5% of the isolates were MRSA. Molecular analysis revealed that 7nuc genes specific for S. aureus from 20 presumptive MRSA assay were all mecA PCR negative. The isolates were sensitive to gentamicin and ciprofloxacin but proved resistant to cefoxitin and oxacillin. CONCLUSION: High isolation rate of MRSA was found in hunting dogs. Significant level (p<0.05) of MRSA was isolated in the nasal cavity of hunting dogs than its perineum. Only nuc genes were detected from the MRSA isolates. Veterinary World 2016-05 2016-05-24 /pmc/articles/PMC4893722/ /pubmed/27284227 http://dx.doi.org/10.14202/vetworld.2016.501-506 Text en Copyright: © Mustapha, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mustapha, Muhammad
Bukar-Kolo, Yachilla Maryam
Geidam, Yaqub Ahmed
Gulani, Isa Adamu
Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria
title Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria
title_full Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria
title_fullStr Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria
title_full_unstemmed Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria
title_short Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria
title_sort phenotypic and genotypic detection of methicillin-resistant staphylococcus aureus in hunting dogs in maiduguri metropolitan, borno state, nigeria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893722/
https://www.ncbi.nlm.nih.gov/pubmed/27284227
http://dx.doi.org/10.14202/vetworld.2016.501-506
work_keys_str_mv AT mustaphamuhammad phenotypicandgenotypicdetectionofmethicillinresistantstaphylococcusaureusinhuntingdogsinmaidugurimetropolitanbornostatenigeria
AT bukarkoloyachillamaryam phenotypicandgenotypicdetectionofmethicillinresistantstaphylococcusaureusinhuntingdogsinmaidugurimetropolitanbornostatenigeria
AT geidamyaqubahmed phenotypicandgenotypicdetectionofmethicillinresistantstaphylococcusaureusinhuntingdogsinmaidugurimetropolitanbornostatenigeria
AT gulaniisaadamu phenotypicandgenotypicdetectionofmethicillinresistantstaphylococcusaureusinhuntingdogsinmaidugurimetropolitanbornostatenigeria