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Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice

The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse...

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Autores principales: Sala-Vila, Aleix, Navarro-Lérida, Inmaculada, Sánchez-Alvarez, Miguel, Bosch, Marta, Calvo, Carlos, López, Juan Antonio, Calvo, Enrique, Ferguson, Charles, Giacomello, Marta, Serafini, Annalisa, Scorrano, Luca, Enriquez, José Antonio, Balsinde, Jesús, Parton, Robert G., Vázquez, Jesús, Pol, Albert, Del Pozo, Miguel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894368/
https://www.ncbi.nlm.nih.gov/pubmed/27272971
http://dx.doi.org/10.1038/srep27351
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author Sala-Vila, Aleix
Navarro-Lérida, Inmaculada
Sánchez-Alvarez, Miguel
Bosch, Marta
Calvo, Carlos
López, Juan Antonio
Calvo, Enrique
Ferguson, Charles
Giacomello, Marta
Serafini, Annalisa
Scorrano, Luca
Enriquez, José Antonio
Balsinde, Jesús
Parton, Robert G.
Vázquez, Jesús
Pol, Albert
Del Pozo, Miguel A.
author_facet Sala-Vila, Aleix
Navarro-Lérida, Inmaculada
Sánchez-Alvarez, Miguel
Bosch, Marta
Calvo, Carlos
López, Juan Antonio
Calvo, Enrique
Ferguson, Charles
Giacomello, Marta
Serafini, Annalisa
Scorrano, Luca
Enriquez, José Antonio
Balsinde, Jesús
Parton, Robert G.
Vázquez, Jesús
Pol, Albert
Del Pozo, Miguel A.
author_sort Sala-Vila, Aleix
collection PubMed
description The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed.
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spelling pubmed-48943682016-06-10 Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice Sala-Vila, Aleix Navarro-Lérida, Inmaculada Sánchez-Alvarez, Miguel Bosch, Marta Calvo, Carlos López, Juan Antonio Calvo, Enrique Ferguson, Charles Giacomello, Marta Serafini, Annalisa Scorrano, Luca Enriquez, José Antonio Balsinde, Jesús Parton, Robert G. Vázquez, Jesús Pol, Albert Del Pozo, Miguel A. Sci Rep Article The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed. Nature Publishing Group 2016-06-06 /pmc/articles/PMC4894368/ /pubmed/27272971 http://dx.doi.org/10.1038/srep27351 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sala-Vila, Aleix
Navarro-Lérida, Inmaculada
Sánchez-Alvarez, Miguel
Bosch, Marta
Calvo, Carlos
López, Juan Antonio
Calvo, Enrique
Ferguson, Charles
Giacomello, Marta
Serafini, Annalisa
Scorrano, Luca
Enriquez, José Antonio
Balsinde, Jesús
Parton, Robert G.
Vázquez, Jesús
Pol, Albert
Del Pozo, Miguel A.
Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
title Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
title_full Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
title_fullStr Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
title_full_unstemmed Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
title_short Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
title_sort interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894368/
https://www.ncbi.nlm.nih.gov/pubmed/27272971
http://dx.doi.org/10.1038/srep27351
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