Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894450/ https://www.ncbi.nlm.nih.gov/pubmed/25778096 http://dx.doi.org/10.1038/srep09155 |
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author | Wright, Charles S. Banerjee, Shiladitya Iyer-Biswas, Srividya Crosson, Sean Dinner, Aaron R. Scherer, Norbert F. |
author_facet | Wright, Charles S. Banerjee, Shiladitya Iyer-Biswas, Srividya Crosson, Sean Dinner, Aaron R. Scherer, Norbert F. |
author_sort | Wright, Charles S. |
collection | PubMed |
description | We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth, and division of C. crescentus. |
format | Online Article Text |
id | pubmed-4894450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48944502016-06-10 Intergenerational continuity of cell shape dynamics in Caulobacter crescentus Wright, Charles S. Banerjee, Shiladitya Iyer-Biswas, Srividya Crosson, Sean Dinner, Aaron R. Scherer, Norbert F. Sci Rep Article We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth, and division of C. crescentus. Nature Publishing Group 2015-03-17 /pmc/articles/PMC4894450/ /pubmed/25778096 http://dx.doi.org/10.1038/srep09155 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wright, Charles S. Banerjee, Shiladitya Iyer-Biswas, Srividya Crosson, Sean Dinner, Aaron R. Scherer, Norbert F. Intergenerational continuity of cell shape dynamics in Caulobacter crescentus |
title | Intergenerational continuity of cell shape dynamics in Caulobacter crescentus |
title_full | Intergenerational continuity of cell shape dynamics in Caulobacter crescentus |
title_fullStr | Intergenerational continuity of cell shape dynamics in Caulobacter crescentus |
title_full_unstemmed | Intergenerational continuity of cell shape dynamics in Caulobacter crescentus |
title_short | Intergenerational continuity of cell shape dynamics in Caulobacter crescentus |
title_sort | intergenerational continuity of cell shape dynamics in caulobacter crescentus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894450/ https://www.ncbi.nlm.nih.gov/pubmed/25778096 http://dx.doi.org/10.1038/srep09155 |
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