Intergenerational continuity of cell shape dynamics in Caulobacter crescentus

We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements o...

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Autores principales: Wright, Charles S., Banerjee, Shiladitya, Iyer-Biswas, Srividya, Crosson, Sean, Dinner, Aaron R., Scherer, Norbert F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894450/
https://www.ncbi.nlm.nih.gov/pubmed/25778096
http://dx.doi.org/10.1038/srep09155
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author Wright, Charles S.
Banerjee, Shiladitya
Iyer-Biswas, Srividya
Crosson, Sean
Dinner, Aaron R.
Scherer, Norbert F.
author_facet Wright, Charles S.
Banerjee, Shiladitya
Iyer-Biswas, Srividya
Crosson, Sean
Dinner, Aaron R.
Scherer, Norbert F.
author_sort Wright, Charles S.
collection PubMed
description We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth, and division of C. crescentus.
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spelling pubmed-48944502016-06-10 Intergenerational continuity of cell shape dynamics in Caulobacter crescentus Wright, Charles S. Banerjee, Shiladitya Iyer-Biswas, Srividya Crosson, Sean Dinner, Aaron R. Scherer, Norbert F. Sci Rep Article We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth, and division of C. crescentus. Nature Publishing Group 2015-03-17 /pmc/articles/PMC4894450/ /pubmed/25778096 http://dx.doi.org/10.1038/srep09155 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wright, Charles S.
Banerjee, Shiladitya
Iyer-Biswas, Srividya
Crosson, Sean
Dinner, Aaron R.
Scherer, Norbert F.
Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
title Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
title_full Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
title_fullStr Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
title_full_unstemmed Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
title_short Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
title_sort intergenerational continuity of cell shape dynamics in caulobacter crescentus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894450/
https://www.ncbi.nlm.nih.gov/pubmed/25778096
http://dx.doi.org/10.1038/srep09155
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