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Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species

In this study, the complete mitochondrial (mt) genomes of Chrysoporthe austroafricana (190,834 bp), C. cubensis (89,084 bp) and C. deuterocubensis (124,412 bp) were determined. Additionally, the mitochondrial genome of another member of the Cryphonectriaceae, namely Cryphonectria parasitica (158,902...

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Autores principales: Kanzi, Aquillah Mumo, Wingfield, Brenda Diana, Steenkamp, Emma Theodora, Naidoo, Sanushka, van der Merwe, Nicolaas Albertus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894602/
https://www.ncbi.nlm.nih.gov/pubmed/27272523
http://dx.doi.org/10.1371/journal.pone.0156104
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author Kanzi, Aquillah Mumo
Wingfield, Brenda Diana
Steenkamp, Emma Theodora
Naidoo, Sanushka
van der Merwe, Nicolaas Albertus
author_facet Kanzi, Aquillah Mumo
Wingfield, Brenda Diana
Steenkamp, Emma Theodora
Naidoo, Sanushka
van der Merwe, Nicolaas Albertus
author_sort Kanzi, Aquillah Mumo
collection PubMed
description In this study, the complete mitochondrial (mt) genomes of Chrysoporthe austroafricana (190,834 bp), C. cubensis (89,084 bp) and C. deuterocubensis (124,412 bp) were determined. Additionally, the mitochondrial genome of another member of the Cryphonectriaceae, namely Cryphonectria parasitica (158,902 bp), was retrieved and annotated for comparative purposes. These genomes showed high levels of synteny, especially in regions including genes involved in oxidative phosphorylation and electron transfer, unique open reading frames (uORFs), ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs), as well as intron positions. Comparative analyses revealed signatures of duplication events, intron number and length variation, and varying intronic ORFs which highlighted the genetic diversity of mt genomes among the Cryphonectriaceae. These mt genomes showed remarkable size polymorphism. The size polymorphism in the mt genomes of these closely related Chrysoporthe species was attributed to the varying number and length of introns, coding sequences and to a lesser extent, intergenic sequences. Compared to publicly available fungal mt genomes, the C. austroafricana mt genome is the second largest in the Ascomycetes thus far.
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spelling pubmed-48946022016-06-23 Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species Kanzi, Aquillah Mumo Wingfield, Brenda Diana Steenkamp, Emma Theodora Naidoo, Sanushka van der Merwe, Nicolaas Albertus PLoS One Research Article In this study, the complete mitochondrial (mt) genomes of Chrysoporthe austroafricana (190,834 bp), C. cubensis (89,084 bp) and C. deuterocubensis (124,412 bp) were determined. Additionally, the mitochondrial genome of another member of the Cryphonectriaceae, namely Cryphonectria parasitica (158,902 bp), was retrieved and annotated for comparative purposes. These genomes showed high levels of synteny, especially in regions including genes involved in oxidative phosphorylation and electron transfer, unique open reading frames (uORFs), ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs), as well as intron positions. Comparative analyses revealed signatures of duplication events, intron number and length variation, and varying intronic ORFs which highlighted the genetic diversity of mt genomes among the Cryphonectriaceae. These mt genomes showed remarkable size polymorphism. The size polymorphism in the mt genomes of these closely related Chrysoporthe species was attributed to the varying number and length of introns, coding sequences and to a lesser extent, intergenic sequences. Compared to publicly available fungal mt genomes, the C. austroafricana mt genome is the second largest in the Ascomycetes thus far. Public Library of Science 2016-06-06 /pmc/articles/PMC4894602/ /pubmed/27272523 http://dx.doi.org/10.1371/journal.pone.0156104 Text en © 2016 Kanzi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kanzi, Aquillah Mumo
Wingfield, Brenda Diana
Steenkamp, Emma Theodora
Naidoo, Sanushka
van der Merwe, Nicolaas Albertus
Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species
title Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species
title_full Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species
title_fullStr Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species
title_full_unstemmed Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species
title_short Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species
title_sort intron derived size polymorphism in the mitochondrial genomes of closely related chrysoporthe species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894602/
https://www.ncbi.nlm.nih.gov/pubmed/27272523
http://dx.doi.org/10.1371/journal.pone.0156104
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