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Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology

Mycoplasma genitalium is a cause of non-gonoccocal urethritis (NGU) in men and cervicitis and pelvic inflammatory disease in women. Recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for M. genitalium is becoming less effective, with the corresp...

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Autores principales: Tabrizi, Sepehr N., Tan, Lit Y., Walker, Samantha, Twin, Jimmy, Poljak, Marin, Bradshaw, Catriona S., Fairley, Christopher K., Bissessor, Melanie, Mokany, Elisa, Todd, Alison V., Garland, Suzanne M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894623/
https://www.ncbi.nlm.nih.gov/pubmed/27271704
http://dx.doi.org/10.1371/journal.pone.0156740
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author Tabrizi, Sepehr N.
Tan, Lit Y.
Walker, Samantha
Twin, Jimmy
Poljak, Marin
Bradshaw, Catriona S.
Fairley, Christopher K.
Bissessor, Melanie
Mokany, Elisa
Todd, Alison V.
Garland, Suzanne M.
author_facet Tabrizi, Sepehr N.
Tan, Lit Y.
Walker, Samantha
Twin, Jimmy
Poljak, Marin
Bradshaw, Catriona S.
Fairley, Christopher K.
Bissessor, Melanie
Mokany, Elisa
Todd, Alison V.
Garland, Suzanne M.
author_sort Tabrizi, Sepehr N.
collection PubMed
description Mycoplasma genitalium is a cause of non-gonoccocal urethritis (NGU) in men and cervicitis and pelvic inflammatory disease in women. Recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for M. genitalium is becoming less effective, with the corresponding emergence of macrolide resistant strains. Increasing failure rates of azithromycin for M. genitalium has significant implications for the presumptive treatment of NGU and international clinical treatment guidelines. Assays able to predict macrolide resistance along with detection of M. genitalium will be useful to enable appropriate selection of antimicrobials to which the organism is susceptible and facilitate high levels of rapid cure. One such assay recently developed is the MG 23S assay, which employs novel PlexZyme™ and PlexPrime™ technology. It is a multiplex assay for detection of M. genitalium and 5 mutations associated with macrolide resistance. The assay was evaluated in 400 samples from 254 (186 males and 68 females) consecutively infected participants, undergoing tests of cure. Using the MG 23S assay, 83% (331/440) of samples were positive, with 56% of positives carrying a macrolide resistance mutation. Comparison of the MG 23S assay to a reference qPCR method for M. genitalium detection and high resolution melt analysis (HRMA) and sequencing for detection of macrolide resistance mutations, resulted in a sensitivity and specificity for M. genitalium detection and for macrolide resistance of 99.1/98.5% and 97.4/100%, respectively. The MG 23S assay provides a considerable advantage in clinical settings through combined diagnosis and detection of macrolide resistance.
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spelling pubmed-48946232016-06-23 Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology Tabrizi, Sepehr N. Tan, Lit Y. Walker, Samantha Twin, Jimmy Poljak, Marin Bradshaw, Catriona S. Fairley, Christopher K. Bissessor, Melanie Mokany, Elisa Todd, Alison V. Garland, Suzanne M. PLoS One Research Article Mycoplasma genitalium is a cause of non-gonoccocal urethritis (NGU) in men and cervicitis and pelvic inflammatory disease in women. Recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for M. genitalium is becoming less effective, with the corresponding emergence of macrolide resistant strains. Increasing failure rates of azithromycin for M. genitalium has significant implications for the presumptive treatment of NGU and international clinical treatment guidelines. Assays able to predict macrolide resistance along with detection of M. genitalium will be useful to enable appropriate selection of antimicrobials to which the organism is susceptible and facilitate high levels of rapid cure. One such assay recently developed is the MG 23S assay, which employs novel PlexZyme™ and PlexPrime™ technology. It is a multiplex assay for detection of M. genitalium and 5 mutations associated with macrolide resistance. The assay was evaluated in 400 samples from 254 (186 males and 68 females) consecutively infected participants, undergoing tests of cure. Using the MG 23S assay, 83% (331/440) of samples were positive, with 56% of positives carrying a macrolide resistance mutation. Comparison of the MG 23S assay to a reference qPCR method for M. genitalium detection and high resolution melt analysis (HRMA) and sequencing for detection of macrolide resistance mutations, resulted in a sensitivity and specificity for M. genitalium detection and for macrolide resistance of 99.1/98.5% and 97.4/100%, respectively. The MG 23S assay provides a considerable advantage in clinical settings through combined diagnosis and detection of macrolide resistance. Public Library of Science 2016-06-06 /pmc/articles/PMC4894623/ /pubmed/27271704 http://dx.doi.org/10.1371/journal.pone.0156740 Text en © 2016 Tabrizi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tabrizi, Sepehr N.
Tan, Lit Y.
Walker, Samantha
Twin, Jimmy
Poljak, Marin
Bradshaw, Catriona S.
Fairley, Christopher K.
Bissessor, Melanie
Mokany, Elisa
Todd, Alison V.
Garland, Suzanne M.
Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
title Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
title_full Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
title_fullStr Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
title_full_unstemmed Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
title_short Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
title_sort multiplex assay for simultaneous detection of mycoplasma genitalium and macrolide resistance using plexzyme and plexprime technology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894623/
https://www.ncbi.nlm.nih.gov/pubmed/27271704
http://dx.doi.org/10.1371/journal.pone.0156740
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