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Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma

Non-thermal atmospheric pressure plasma has attracted great interest due to its multiple potential biomedical applications with cancer treatment being among the most urgent. To realize the clinical potential of non-thermal plasma, the exact cellular and molecular mechanisms of plasma effects must be...

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Autores principales: Zhunussova, Aigul, Vitol, Elina A., Polyak, Boris, Tuleukhanov, Sultan, Brooks, Ari D., Sensenig, Richard, Friedman, Gary, Orynbayeva, Zulfiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894638/
https://www.ncbi.nlm.nih.gov/pubmed/27270230
http://dx.doi.org/10.1371/journal.pone.0156818
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author Zhunussova, Aigul
Vitol, Elina A.
Polyak, Boris
Tuleukhanov, Sultan
Brooks, Ari D.
Sensenig, Richard
Friedman, Gary
Orynbayeva, Zulfiya
author_facet Zhunussova, Aigul
Vitol, Elina A.
Polyak, Boris
Tuleukhanov, Sultan
Brooks, Ari D.
Sensenig, Richard
Friedman, Gary
Orynbayeva, Zulfiya
author_sort Zhunussova, Aigul
collection PubMed
description Non-thermal atmospheric pressure plasma has attracted great interest due to its multiple potential biomedical applications with cancer treatment being among the most urgent. To realize the clinical potential of non-thermal plasma, the exact cellular and molecular mechanisms of plasma effects must be understood. This work aimed at studying the prostate cancer specific mechanisms of non-thermal plasma effects on energy metabolism as a central regulator of cell homeostasis and proliferation. It was found that cancer cells with higher metabolic rate initially are more resistant to plasma treated phosphate-buffered saline (PBS) since the respiratory and calcium sensitive signaling systems were not responsive to plasma exposure. However, dramatic decline of cancer oxidative phosphorylation developed over time resulted in significant progression of cell lethality. The normal prostate cells with low metabolic activity immediately responded to plasma treated PBS by suppression of respiratory functions and sustained elevation of cytosolic calcium. However, over time the normal cells start recovering their mitochondria functions, proliferate and restore the cell population. We found that the non-thermal plasma induced increase in intracellular ROS is of primarily non-mitochondrial origin. The discriminate non-thermal plasma effects hold a promise for clinical cancer intervention.
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spelling pubmed-48946382016-06-23 Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma Zhunussova, Aigul Vitol, Elina A. Polyak, Boris Tuleukhanov, Sultan Brooks, Ari D. Sensenig, Richard Friedman, Gary Orynbayeva, Zulfiya PLoS One Research Article Non-thermal atmospheric pressure plasma has attracted great interest due to its multiple potential biomedical applications with cancer treatment being among the most urgent. To realize the clinical potential of non-thermal plasma, the exact cellular and molecular mechanisms of plasma effects must be understood. This work aimed at studying the prostate cancer specific mechanisms of non-thermal plasma effects on energy metabolism as a central regulator of cell homeostasis and proliferation. It was found that cancer cells with higher metabolic rate initially are more resistant to plasma treated phosphate-buffered saline (PBS) since the respiratory and calcium sensitive signaling systems were not responsive to plasma exposure. However, dramatic decline of cancer oxidative phosphorylation developed over time resulted in significant progression of cell lethality. The normal prostate cells with low metabolic activity immediately responded to plasma treated PBS by suppression of respiratory functions and sustained elevation of cytosolic calcium. However, over time the normal cells start recovering their mitochondria functions, proliferate and restore the cell population. We found that the non-thermal plasma induced increase in intracellular ROS is of primarily non-mitochondrial origin. The discriminate non-thermal plasma effects hold a promise for clinical cancer intervention. Public Library of Science 2016-06-06 /pmc/articles/PMC4894638/ /pubmed/27270230 http://dx.doi.org/10.1371/journal.pone.0156818 Text en © 2016 Zhunussova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhunussova, Aigul
Vitol, Elina A.
Polyak, Boris
Tuleukhanov, Sultan
Brooks, Ari D.
Sensenig, Richard
Friedman, Gary
Orynbayeva, Zulfiya
Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma
title Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma
title_full Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma
title_fullStr Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma
title_full_unstemmed Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma
title_short Mitochondria-Mediated Anticancer Effects of Non-Thermal Atmospheric Plasma
title_sort mitochondria-mediated anticancer effects of non-thermal atmospheric plasma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894638/
https://www.ncbi.nlm.nih.gov/pubmed/27270230
http://dx.doi.org/10.1371/journal.pone.0156818
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