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Programmable DNA Nanosystem for Molecular Interrogation

We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core, and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements who...

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Detalles Bibliográficos
Autores principales: Mathur, Divita, Henderson, Eric R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895238/
https://www.ncbi.nlm.nih.gov/pubmed/27270162
http://dx.doi.org/10.1038/srep27413
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author Mathur, Divita
Henderson, Eric R.
author_facet Mathur, Divita
Henderson, Eric R.
author_sort Mathur, Divita
collection PubMed
description We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core, and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force-generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame thereby providing a mechanism for molecular detection and interrogation at the ring-frame interface. As proof of principle we use this system to evaluate base stacking adhesion and demonstrate detection of a soluble nucleic acid viral genome mimic.
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spelling pubmed-48952382016-06-10 Programmable DNA Nanosystem for Molecular Interrogation Mathur, Divita Henderson, Eric R. Sci Rep Article We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core, and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force-generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame thereby providing a mechanism for molecular detection and interrogation at the ring-frame interface. As proof of principle we use this system to evaluate base stacking adhesion and demonstrate detection of a soluble nucleic acid viral genome mimic. Nature Publishing Group 2016-06-07 /pmc/articles/PMC4895238/ /pubmed/27270162 http://dx.doi.org/10.1038/srep27413 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mathur, Divita
Henderson, Eric R.
Programmable DNA Nanosystem for Molecular Interrogation
title Programmable DNA Nanosystem for Molecular Interrogation
title_full Programmable DNA Nanosystem for Molecular Interrogation
title_fullStr Programmable DNA Nanosystem for Molecular Interrogation
title_full_unstemmed Programmable DNA Nanosystem for Molecular Interrogation
title_short Programmable DNA Nanosystem for Molecular Interrogation
title_sort programmable dna nanosystem for molecular interrogation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895238/
https://www.ncbi.nlm.nih.gov/pubmed/27270162
http://dx.doi.org/10.1038/srep27413
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