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Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging

[Image: see text] Colony biofilms of Bacillus subtilis are a widely used model for studying cellular differentiation. Here, we applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to examine cellular and molecular heterogeneity in B. subtilis colony biofilms. F...

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Autores principales: Si, Tong, Li, Bin, Zhang, Ke, Xu, Yiran, Zhao, Huimin, Sweedler, Jonathan V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895246/
https://www.ncbi.nlm.nih.gov/pubmed/27136705
http://dx.doi.org/10.1021/acs.jproteome.6b00127
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author Si, Tong
Li, Bin
Zhang, Ke
Xu, Yiran
Zhao, Huimin
Sweedler, Jonathan V.
author_facet Si, Tong
Li, Bin
Zhang, Ke
Xu, Yiran
Zhao, Huimin
Sweedler, Jonathan V.
author_sort Si, Tong
collection PubMed
description [Image: see text] Colony biofilms of Bacillus subtilis are a widely used model for studying cellular differentiation. Here, we applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to examine cellular and molecular heterogeneity in B. subtilis colony biofilms. From B. subtilis cells cultivated on a biofilm-promoting medium, we detected two cannibalistic factors not found in previous MALDI MSI studies of the same strain under different culturing conditions. Given the importance of cannibalism in matrix formation of B. subtilis biofilms, we employed a transcriptional reporter to monitor matrix-producing cell subpopulations using fluorescence imaging. These two complementary imaging approaches were used to characterize three B. subtilis strains, the wild type isolate NCIB3610, and two mutants, Δspo0A and ΔabrB, with defective and enhanced biofilm phenotypes, respectively. Upon deletion of key transcriptional factors, correlated changes were observed in biofilm morphology, signaling, cannibalistic factor distribution, and matrix-related gene expression, providing new insights on cannibalism in biofilm development. This work underscores the advantages of using multimodal imaging to compare spatial patterns of selected molecules with the associated protein expression patterns, obtaining information on cellular heterogeneity and function not obtainable when using a single method to characterize biofilm formation.
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spelling pubmed-48952462016-06-08 Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging Si, Tong Li, Bin Zhang, Ke Xu, Yiran Zhao, Huimin Sweedler, Jonathan V. J Proteome Res [Image: see text] Colony biofilms of Bacillus subtilis are a widely used model for studying cellular differentiation. Here, we applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to examine cellular and molecular heterogeneity in B. subtilis colony biofilms. From B. subtilis cells cultivated on a biofilm-promoting medium, we detected two cannibalistic factors not found in previous MALDI MSI studies of the same strain under different culturing conditions. Given the importance of cannibalism in matrix formation of B. subtilis biofilms, we employed a transcriptional reporter to monitor matrix-producing cell subpopulations using fluorescence imaging. These two complementary imaging approaches were used to characterize three B. subtilis strains, the wild type isolate NCIB3610, and two mutants, Δspo0A and ΔabrB, with defective and enhanced biofilm phenotypes, respectively. Upon deletion of key transcriptional factors, correlated changes were observed in biofilm morphology, signaling, cannibalistic factor distribution, and matrix-related gene expression, providing new insights on cannibalism in biofilm development. This work underscores the advantages of using multimodal imaging to compare spatial patterns of selected molecules with the associated protein expression patterns, obtaining information on cellular heterogeneity and function not obtainable when using a single method to characterize biofilm formation. American Chemical Society 2016-05-03 2016-06-03 /pmc/articles/PMC4895246/ /pubmed/27136705 http://dx.doi.org/10.1021/acs.jproteome.6b00127 Text en Copyright © 2016 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Si, Tong
Li, Bin
Zhang, Ke
Xu, Yiran
Zhao, Huimin
Sweedler, Jonathan V.
Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging
title Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging
title_full Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging
title_fullStr Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging
title_full_unstemmed Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging
title_short Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging
title_sort characterization of bacillus subtilis colony biofilms via mass spectrometry and fluorescence imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895246/
https://www.ncbi.nlm.nih.gov/pubmed/27136705
http://dx.doi.org/10.1021/acs.jproteome.6b00127
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