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Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients
Emerging evidence implicates platelets as key mediators of venous thromboembolism (VTE). Nevertheless, the pathways by which platelets and circulating procoagulant proteins synergistically orchestrate VTE remain incompletely understood. We prospectively determined whether activated platelets and sys...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895334/ https://www.ncbi.nlm.nih.gov/pubmed/27270163 http://dx.doi.org/10.1038/srep27478 |
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author | Shih, Lauren Kaplan, David Kraiss, Larry W. Casper, T. Charles Pendleton, Robert C. Peters, Christopher L. Supiano, Mark A. Zimmerman, Guy A. Weyrich, Andrew S. Rondina, Matthew T. |
author_facet | Shih, Lauren Kaplan, David Kraiss, Larry W. Casper, T. Charles Pendleton, Robert C. Peters, Christopher L. Supiano, Mark A. Zimmerman, Guy A. Weyrich, Andrew S. Rondina, Matthew T. |
author_sort | Shih, Lauren |
collection | PubMed |
description | Emerging evidence implicates platelets as key mediators of venous thromboembolism (VTE). Nevertheless, the pathways by which platelets and circulating procoagulant proteins synergistically orchestrate VTE remain incompletely understood. We prospectively determined whether activated platelets and systemic procoagulant factors were associated with VTE in 32 older orthopedic surgery patients. Circulating platelet-monocyte aggregates (PMAs), p-selectin expression (P-SEL), and integrin αIIbβ3 activation (PAC-1 binding) were assessed pre-operatively and 24 hours post-operatively. The proinflammatory and procoagulant molecule C-reactive protein (CRP), which induces PMA formation in vitro, along with plasma d-dimer and fibrinogen levels were also measured. The primary outcome was VTE occurring within 30 days post-operatively. Overall, 40.6% of patients developed VTE. Patients with VTE had a significant increase in circulating PMAs and CRP post-operatively, compared to those without VTE. Changes in PMA and CRP in VTE patients were significantly correlated (r(2) = 0.536, p = 0.004). In contrast, P-SEL expression and PAC-1 binding, fibrinogen levels, and d-dimers were not associated with VTE. This is the first study to identify that increased circulating PMAs and CRP levels are early markers associated with post-surgical VTE. Our findings also provide new clinical evidence supporting the interplay between PMAs and CRP in patients with VTE. |
format | Online Article Text |
id | pubmed-4895334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48953342016-06-10 Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients Shih, Lauren Kaplan, David Kraiss, Larry W. Casper, T. Charles Pendleton, Robert C. Peters, Christopher L. Supiano, Mark A. Zimmerman, Guy A. Weyrich, Andrew S. Rondina, Matthew T. Sci Rep Article Emerging evidence implicates platelets as key mediators of venous thromboembolism (VTE). Nevertheless, the pathways by which platelets and circulating procoagulant proteins synergistically orchestrate VTE remain incompletely understood. We prospectively determined whether activated platelets and systemic procoagulant factors were associated with VTE in 32 older orthopedic surgery patients. Circulating platelet-monocyte aggregates (PMAs), p-selectin expression (P-SEL), and integrin αIIbβ3 activation (PAC-1 binding) were assessed pre-operatively and 24 hours post-operatively. The proinflammatory and procoagulant molecule C-reactive protein (CRP), which induces PMA formation in vitro, along with plasma d-dimer and fibrinogen levels were also measured. The primary outcome was VTE occurring within 30 days post-operatively. Overall, 40.6% of patients developed VTE. Patients with VTE had a significant increase in circulating PMAs and CRP post-operatively, compared to those without VTE. Changes in PMA and CRP in VTE patients were significantly correlated (r(2) = 0.536, p = 0.004). In contrast, P-SEL expression and PAC-1 binding, fibrinogen levels, and d-dimers were not associated with VTE. This is the first study to identify that increased circulating PMAs and CRP levels are early markers associated with post-surgical VTE. Our findings also provide new clinical evidence supporting the interplay between PMAs and CRP in patients with VTE. Nature Publishing Group 2016-06-07 /pmc/articles/PMC4895334/ /pubmed/27270163 http://dx.doi.org/10.1038/srep27478 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Shih, Lauren Kaplan, David Kraiss, Larry W. Casper, T. Charles Pendleton, Robert C. Peters, Christopher L. Supiano, Mark A. Zimmerman, Guy A. Weyrich, Andrew S. Rondina, Matthew T. Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients |
title | Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients |
title_full | Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients |
title_fullStr | Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients |
title_full_unstemmed | Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients |
title_short | Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients |
title_sort | platelet-monocyte aggregates and c-reactive protein are associated with vte in older surgical patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895334/ https://www.ncbi.nlm.nih.gov/pubmed/27270163 http://dx.doi.org/10.1038/srep27478 |
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