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An Unroofing Method to Observe the Cytoskeleton Directly at Molecular Resolution Using Atomic Force Microscopy
An improved unroofing method enabled the cantilever of an atomic force microscope (AFM) to reach directly into a cell to visualize the intracellular cytoskeletal actin filaments, microtubules, clathrin coats, and caveolae in phosphate-buffered saline (PBS) at a higher resolution than conventional el...
Autores principales: | Usukura, Eiji, Narita, Akihiro, Yagi, Akira, Ito, Shuichi, Usukura, Jiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895337/ https://www.ncbi.nlm.nih.gov/pubmed/27273367 http://dx.doi.org/10.1038/srep27472 |
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