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aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis
Epithelial cells exhibit apical membrane protrusions, which confer specific functions to epithelial tissues. Microridges are short actin protrusions that are laterally long and form a maze-like pattern in the apical domain. They are widely found on vertebrate squamous epithelia including epidermis a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895443/ https://www.ncbi.nlm.nih.gov/pubmed/27249668 http://dx.doi.org/10.1038/ncomms11643 |
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author | Raman, Renuka Damle, Indraneel Rote, Rahul Banerjee, Shamik Dingare, Chaitanya Sonawane, Mahendra |
author_facet | Raman, Renuka Damle, Indraneel Rote, Rahul Banerjee, Shamik Dingare, Chaitanya Sonawane, Mahendra |
author_sort | Raman, Renuka |
collection | PubMed |
description | Epithelial cells exhibit apical membrane protrusions, which confer specific functions to epithelial tissues. Microridges are short actin protrusions that are laterally long and form a maze-like pattern in the apical domain. They are widely found on vertebrate squamous epithelia including epidermis and have functions in mucous retention, membrane storage and abrasion resistance. It is largely unknown how the formation of these laterally long actin projections is regulated. Here, we show that antagonistic interactions between aPKC and Lgl–regulators of apical and basolateral domain identity, respectively,–control the length of microridges in the zebrafish periderm, the outermost layer of the epidermis. aPKC regulates the levels of Lgl and the active form of non-muscle myosinII at the apical cortex to prevent actin polymerization-dependent precocious fusion and elongation of microridges. Our data unravels the functional significance of exclusion of Lgl from the apical domain in epithelial cells. |
format | Online Article Text |
id | pubmed-4895443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48954432016-06-21 aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis Raman, Renuka Damle, Indraneel Rote, Rahul Banerjee, Shamik Dingare, Chaitanya Sonawane, Mahendra Nat Commun Article Epithelial cells exhibit apical membrane protrusions, which confer specific functions to epithelial tissues. Microridges are short actin protrusions that are laterally long and form a maze-like pattern in the apical domain. They are widely found on vertebrate squamous epithelia including epidermis and have functions in mucous retention, membrane storage and abrasion resistance. It is largely unknown how the formation of these laterally long actin projections is regulated. Here, we show that antagonistic interactions between aPKC and Lgl–regulators of apical and basolateral domain identity, respectively,–control the length of microridges in the zebrafish periderm, the outermost layer of the epidermis. aPKC regulates the levels of Lgl and the active form of non-muscle myosinII at the apical cortex to prevent actin polymerization-dependent precocious fusion and elongation of microridges. Our data unravels the functional significance of exclusion of Lgl from the apical domain in epithelial cells. Nature Publishing Group 2016-06-01 /pmc/articles/PMC4895443/ /pubmed/27249668 http://dx.doi.org/10.1038/ncomms11643 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Raman, Renuka Damle, Indraneel Rote, Rahul Banerjee, Shamik Dingare, Chaitanya Sonawane, Mahendra aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis |
title | aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis |
title_full | aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis |
title_fullStr | aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis |
title_full_unstemmed | aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis |
title_short | aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis |
title_sort | apkc regulates apical localization of lgl to restrict elongation of microridges in developing zebrafish epidermis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895443/ https://www.ncbi.nlm.nih.gov/pubmed/27249668 http://dx.doi.org/10.1038/ncomms11643 |
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