Cargando…

Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs

BACKGROUND: Dogs with congenital portosystemic shunts (CPSS) have hypoplasia of the intrahepatic portal veins. Surgical CPSS attenuation results in the development of the intrahepatic portal vasculature, the precise mechanism for which is unknown, although new vessel formation by angiogenesis is sus...

Descripción completa

Detalles Bibliográficos
Autores principales: Tivers, M.S., House, A.K., Smith, K.C., Wheeler‐Jones, C.P.D., Lipscomb, V.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895587/
https://www.ncbi.nlm.nih.gov/pubmed/25132501
http://dx.doi.org/10.1111/jvim.12411
_version_ 1782435880203452416
author Tivers, M.S.
House, A.K.
Smith, K.C.
Wheeler‐Jones, C.P.D.
Lipscomb, V.J.
author_facet Tivers, M.S.
House, A.K.
Smith, K.C.
Wheeler‐Jones, C.P.D.
Lipscomb, V.J.
author_sort Tivers, M.S.
collection PubMed
description BACKGROUND: Dogs with congenital portosystemic shunts (CPSS) have hypoplasia of the intrahepatic portal veins. Surgical CPSS attenuation results in the development of the intrahepatic portal vasculature, the precise mechanism for which is unknown, although new vessel formation by angiogenesis is suspected. HYPOTHESIS: That the degree of portal vascular development and the increase in portal vascularization after CPSS attenuation is significantly associated with hepatic vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR2) gene expression and serum VEGF concentration. ANIMALS: Client‐owned dogs with CPSS undergoing surgical treatment. Forty‐nine dogs were included in the gene expression data and 35 in the serum VEGF data. MATERIALS AND METHODS: Dogs surgically treated by partial or complete CPSS attenuation were prospectively recruited. Relative gene expression of VEGF and VEGFR2 was measured in liver biopsy samples taken at initial and follow‐up surgery using quantitative polymerase chain reaction. Serum VEGF concentration was measured before and after CPSS attenuation using a canine specific ELISA. Statistical significance was set at the 5% level (P ≤ .05). RESULTS: There was a significant increase in the mRNA expression of VEGFR2 after partial attenuation (P = .006). Dogs that could tolerate complete attenuation had significantly greater VEGFR2 mRNA expression than those that only tolerated partial attenuation (P = .037). Serum VEGF concentration was significantly increased at 24 (P < .001) and 48 (P = .003) hours after attenuation. CONCLUSIONS AND CLINICAL IMPORTANCE: These findings suggest that intrahepatic angiogenesis is likely to occur after the surgical attenuation of CPSS in dogs, and contributes to the development of the intrahepatic vasculature postoperatively.
format Online
Article
Text
id pubmed-4895587
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-48955872016-06-22 Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs Tivers, M.S. House, A.K. Smith, K.C. Wheeler‐Jones, C.P.D. Lipscomb, V.J. J Vet Intern Med Standard Articles BACKGROUND: Dogs with congenital portosystemic shunts (CPSS) have hypoplasia of the intrahepatic portal veins. Surgical CPSS attenuation results in the development of the intrahepatic portal vasculature, the precise mechanism for which is unknown, although new vessel formation by angiogenesis is suspected. HYPOTHESIS: That the degree of portal vascular development and the increase in portal vascularization after CPSS attenuation is significantly associated with hepatic vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR2) gene expression and serum VEGF concentration. ANIMALS: Client‐owned dogs with CPSS undergoing surgical treatment. Forty‐nine dogs were included in the gene expression data and 35 in the serum VEGF data. MATERIALS AND METHODS: Dogs surgically treated by partial or complete CPSS attenuation were prospectively recruited. Relative gene expression of VEGF and VEGFR2 was measured in liver biopsy samples taken at initial and follow‐up surgery using quantitative polymerase chain reaction. Serum VEGF concentration was measured before and after CPSS attenuation using a canine specific ELISA. Statistical significance was set at the 5% level (P ≤ .05). RESULTS: There was a significant increase in the mRNA expression of VEGFR2 after partial attenuation (P = .006). Dogs that could tolerate complete attenuation had significantly greater VEGFR2 mRNA expression than those that only tolerated partial attenuation (P = .037). Serum VEGF concentration was significantly increased at 24 (P < .001) and 48 (P = .003) hours after attenuation. CONCLUSIONS AND CLINICAL IMPORTANCE: These findings suggest that intrahepatic angiogenesis is likely to occur after the surgical attenuation of CPSS in dogs, and contributes to the development of the intrahepatic vasculature postoperatively. John Wiley and Sons Inc. 2014-08-08 2014 /pmc/articles/PMC4895587/ /pubmed/25132501 http://dx.doi.org/10.1111/jvim.12411 Text en Copyright © 2014 by the American College of Veterinary Internal Medicine
spellingShingle Standard Articles
Tivers, M.S.
House, A.K.
Smith, K.C.
Wheeler‐Jones, C.P.D.
Lipscomb, V.J.
Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs
title Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs
title_full Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs
title_fullStr Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs
title_full_unstemmed Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs
title_short Markers of Angiogenesis Associated with Surgical Attenuation of Congenital Portosystemic Shunts in Dogs
title_sort markers of angiogenesis associated with surgical attenuation of congenital portosystemic shunts in dogs
topic Standard Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895587/
https://www.ncbi.nlm.nih.gov/pubmed/25132501
http://dx.doi.org/10.1111/jvim.12411
work_keys_str_mv AT tiversms markersofangiogenesisassociatedwithsurgicalattenuationofcongenitalportosystemicshuntsindogs
AT houseak markersofangiogenesisassociatedwithsurgicalattenuationofcongenitalportosystemicshuntsindogs
AT smithkc markersofangiogenesisassociatedwithsurgicalattenuationofcongenitalportosystemicshuntsindogs
AT wheelerjonescpd markersofangiogenesisassociatedwithsurgicalattenuationofcongenitalportosystemicshuntsindogs
AT lipscombvj markersofangiogenesisassociatedwithsurgicalattenuationofcongenitalportosystemicshuntsindogs