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Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests
BACKGROUND: Salmonella enterica can significantly impact management of animal facilities. Comprehensive screening is essential for effective control in high‐risk populations. Availability of reliable point‐of‐care diagnostic tests would facilitate these efforts. HYPOTHESIS/OBJECTIVES: Compare the ab...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895624/ https://www.ncbi.nlm.nih.gov/pubmed/25312330 http://dx.doi.org/10.1111/jvim.12440 |
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author | Burgess, B.A. Weller, C.B. Pabilonia, K.L. Bolte, D.S. Van Metre, D.C. Morley, P.S. |
author_facet | Burgess, B.A. Weller, C.B. Pabilonia, K.L. Bolte, D.S. Van Metre, D.C. Morley, P.S. |
author_sort | Burgess, B.A. |
collection | PubMed |
description | BACKGROUND: Salmonella enterica can significantly impact management of animal facilities. Comprehensive screening is essential for effective control in high‐risk populations. Availability of reliable point‐of‐care diagnostic tests would facilitate these efforts. HYPOTHESIS/OBJECTIVES: Compare the ability of commercially available rapid diagnostic assays (2 lateral flow immunoassays [LFIs], DNA hybridization [DNAH], real‐time PCR [qPCR]), and culture to detect common serotypes of S. enterica in feces. ANIMALS: n/a. METHODS: In an experimental study, 112 S. enterica isolates were randomly selected from the 10 most common serotypes recovered at a veterinary hospital. Archived isolates were amplified in broth and standardized inocula (100 colony forming units) were incubated with equine feces in tetrathionate broth (TET). Cultures were tested in a blinded fashion by using LFIs, DNAH, qPCR, and culture. RESULTS: The LFIs detected 84% and 67% of isolates, respectively, but reactivity varied among serotypes. Both reacted poorly with serotype Cerro (Group K) isolates, and 1 LFI did not react with any serotype Mbandaka (Group C1) or Montevideo (Group C1) isolates. DNAH detected 94% of isolates, whereas culture and qPCR most reliably detected all serotypes. False‐positive results were obtained for 4 negative controls by using DNAH and 1 negative control by using qPCR, but LFIs and culture had no false‐positive results. CONCLUSIONS AND CLINICAL IMPORTANCE: Culture, qPCR, and DNAH were effective in detecting most Salmonella isolates, but have limited application at point‐of‐care settings. LFIs are appealing as point‐of‐care tests because of low cost and ease of use, but limited detection of some serotypes needs to be evaluated with samples obtained from naturally infected animals. |
format | Online Article Text |
id | pubmed-4895624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48956242016-06-22 Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests Burgess, B.A. Weller, C.B. Pabilonia, K.L. Bolte, D.S. Van Metre, D.C. Morley, P.S. J Vet Intern Med Standard Articles BACKGROUND: Salmonella enterica can significantly impact management of animal facilities. Comprehensive screening is essential for effective control in high‐risk populations. Availability of reliable point‐of‐care diagnostic tests would facilitate these efforts. HYPOTHESIS/OBJECTIVES: Compare the ability of commercially available rapid diagnostic assays (2 lateral flow immunoassays [LFIs], DNA hybridization [DNAH], real‐time PCR [qPCR]), and culture to detect common serotypes of S. enterica in feces. ANIMALS: n/a. METHODS: In an experimental study, 112 S. enterica isolates were randomly selected from the 10 most common serotypes recovered at a veterinary hospital. Archived isolates were amplified in broth and standardized inocula (100 colony forming units) were incubated with equine feces in tetrathionate broth (TET). Cultures were tested in a blinded fashion by using LFIs, DNAH, qPCR, and culture. RESULTS: The LFIs detected 84% and 67% of isolates, respectively, but reactivity varied among serotypes. Both reacted poorly with serotype Cerro (Group K) isolates, and 1 LFI did not react with any serotype Mbandaka (Group C1) or Montevideo (Group C1) isolates. DNAH detected 94% of isolates, whereas culture and qPCR most reliably detected all serotypes. False‐positive results were obtained for 4 negative controls by using DNAH and 1 negative control by using qPCR, but LFIs and culture had no false‐positive results. CONCLUSIONS AND CLINICAL IMPORTANCE: Culture, qPCR, and DNAH were effective in detecting most Salmonella isolates, but have limited application at point‐of‐care settings. LFIs are appealing as point‐of‐care tests because of low cost and ease of use, but limited detection of some serotypes needs to be evaluated with samples obtained from naturally infected animals. John Wiley and Sons Inc. 2014-10-13 2014 /pmc/articles/PMC4895624/ /pubmed/25312330 http://dx.doi.org/10.1111/jvim.12440 Text en Copyright © 2014 by the American College of Veterinary Internal Medicine |
spellingShingle | Standard Articles Burgess, B.A. Weller, C.B. Pabilonia, K.L. Bolte, D.S. Van Metre, D.C. Morley, P.S. Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests |
title | Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests |
title_full | Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests |
title_fullStr | Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests |
title_full_unstemmed | Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests |
title_short | Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests |
title_sort | detection of different serotypes of salmonella enterica in experimentally inoculated equine fecal samples by commercially available rapid tests |
topic | Standard Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895624/ https://www.ncbi.nlm.nih.gov/pubmed/25312330 http://dx.doi.org/10.1111/jvim.12440 |
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