Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells

BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that the nonstructural protein 11 (nsp11) of PRRSV may be an important protein for...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Xibao, Zhang, Xiaozhuan, Chang, Yongzhe, Jiang, Bo, Deng, Ruiguang, Wang, Aiping, Zhang, Gaiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895886/
https://www.ncbi.nlm.nih.gov/pubmed/27268206
http://dx.doi.org/10.1186/s12917-016-0717-5
_version_ 1782435943457751040
author Shi, Xibao
Zhang, Xiaozhuan
Chang, Yongzhe
Jiang, Bo
Deng, Ruiguang
Wang, Aiping
Zhang, Gaiping
author_facet Shi, Xibao
Zhang, Xiaozhuan
Chang, Yongzhe
Jiang, Bo
Deng, Ruiguang
Wang, Aiping
Zhang, Gaiping
author_sort Shi, Xibao
collection PubMed
description BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that the nonstructural protein 11 (nsp11) of PRRSV may be an important protein for the immune escape of PRRSV. RESULTS: Here, we firstly explored the effect of over-expression of nsp11 on PRRSV infection and found that over-expression of nsp11 enhanced the PRRSV titers while the small interfering RNA (siRNAs) specifically targeting nsp11 could reduce the PRRSV titers in MARC-145 cells. CONCLUSION: In conclusion, PRRSV nsp11 promotes PRRSV infection in MARC-145 cells and siRNAs targeting nsp11 may be a potential therapeutic strategy to control PRRSV in future.
format Online
Article
Text
id pubmed-4895886
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-48958862016-06-08 Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells Shi, Xibao Zhang, Xiaozhuan Chang, Yongzhe Jiang, Bo Deng, Ruiguang Wang, Aiping Zhang, Gaiping BMC Vet Res Research Article BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that the nonstructural protein 11 (nsp11) of PRRSV may be an important protein for the immune escape of PRRSV. RESULTS: Here, we firstly explored the effect of over-expression of nsp11 on PRRSV infection and found that over-expression of nsp11 enhanced the PRRSV titers while the small interfering RNA (siRNAs) specifically targeting nsp11 could reduce the PRRSV titers in MARC-145 cells. CONCLUSION: In conclusion, PRRSV nsp11 promotes PRRSV infection in MARC-145 cells and siRNAs targeting nsp11 may be a potential therapeutic strategy to control PRRSV in future. BioMed Central 2016-06-06 /pmc/articles/PMC4895886/ /pubmed/27268206 http://dx.doi.org/10.1186/s12917-016-0717-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Shi, Xibao
Zhang, Xiaozhuan
Chang, Yongzhe
Jiang, Bo
Deng, Ruiguang
Wang, Aiping
Zhang, Gaiping
Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
title Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
title_full Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
title_fullStr Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
title_full_unstemmed Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
title_short Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells
title_sort nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (prrsv) promotes prrsv infection in marc-145 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895886/
https://www.ncbi.nlm.nih.gov/pubmed/27268206
http://dx.doi.org/10.1186/s12917-016-0717-5
work_keys_str_mv AT shixibao nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells
AT zhangxiaozhuan nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells
AT changyongzhe nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells
AT jiangbo nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells
AT dengruiguang nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells
AT wangaiping nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells
AT zhanggaiping nonstructuralprotein11nsp11ofporcinereproductiveandrespiratorysyndromevirusprrsvpromotesprrsvinfectioninmarc145cells

Ejemplares similares