Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography

A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow ra...

Descripción completa

Detalles Bibliográficos
Autores principales: Gottumukkala, Raju V. S. S., Nadimpalli, Nareshraju, Sukala, Kannababu, Subbaraju, Gottumukkala V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897191/
https://www.ncbi.nlm.nih.gov/pubmed/27379336
http://dx.doi.org/10.1155/2014/628196
Descripción
Sumario:A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow rate of 1.0 mL/minute with an operating temperature of 30°C and detection with a UV-Visible detector was at 280 nm. The method was validated for linearity, precision, intra- and interday precision, and accuracy. The developed method is successfully applied for the determination of catechin and epicatechin content in chocolates. The Godiva brand chocolate contains high concentration of epicatechin.

Ejemplares similares