Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography

A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow ra...

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Autores principales: Gottumukkala, Raju V. S. S., Nadimpalli, Nareshraju, Sukala, Kannababu, Subbaraju, Gottumukkala V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897191/
https://www.ncbi.nlm.nih.gov/pubmed/27379336
http://dx.doi.org/10.1155/2014/628196
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author Gottumukkala, Raju V. S. S.
Nadimpalli, Nareshraju
Sukala, Kannababu
Subbaraju, Gottumukkala V.
author_facet Gottumukkala, Raju V. S. S.
Nadimpalli, Nareshraju
Sukala, Kannababu
Subbaraju, Gottumukkala V.
author_sort Gottumukkala, Raju V. S. S.
collection PubMed
description A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow rate of 1.0 mL/minute with an operating temperature of 30°C and detection with a UV-Visible detector was at 280 nm. The method was validated for linearity, precision, intra- and interday precision, and accuracy. The developed method is successfully applied for the determination of catechin and epicatechin content in chocolates. The Godiva brand chocolate contains high concentration of epicatechin.
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spelling pubmed-48971912016-07-04 Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography Gottumukkala, Raju V. S. S. Nadimpalli, Nareshraju Sukala, Kannababu Subbaraju, Gottumukkala V. Int Sch Res Notices Research Article A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow rate of 1.0 mL/minute with an operating temperature of 30°C and detection with a UV-Visible detector was at 280 nm. The method was validated for linearity, precision, intra- and interday precision, and accuracy. The developed method is successfully applied for the determination of catechin and epicatechin content in chocolates. The Godiva brand chocolate contains high concentration of epicatechin. Hindawi Publishing Corporation 2014-10-28 /pmc/articles/PMC4897191/ /pubmed/27379336 http://dx.doi.org/10.1155/2014/628196 Text en Copyright © 2014 Raju V. S. S. Gottumukkala et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gottumukkala, Raju V. S. S.
Nadimpalli, Nareshraju
Sukala, Kannababu
Subbaraju, Gottumukkala V.
Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography
title Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography
title_full Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography
title_fullStr Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography
title_full_unstemmed Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography
title_short Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography
title_sort determination of catechin and epicatechin content in chocolates by high-performance liquid chromatography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897191/
https://www.ncbi.nlm.nih.gov/pubmed/27379336
http://dx.doi.org/10.1155/2014/628196
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