Cargando…
Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography
A selective ultraperformance liquid chromatographic (UPLC) method for the determination of magnesium valproate and its process related impurities has been developed. The method includes reversed-phase Acquity BEH C(18) column with 100 mm × 2.1 mm i.d. and 1.7 µ particle size. The mobile phase consis...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897345/ https://www.ncbi.nlm.nih.gov/pubmed/27355082 http://dx.doi.org/10.1155/2014/412704 |
_version_ | 1782436139220598784 |
---|---|
author | Thakkar, Rakshit Saravaia, Hitesh Patel, Madhavi Shah, Anamik |
author_facet | Thakkar, Rakshit Saravaia, Hitesh Patel, Madhavi Shah, Anamik |
author_sort | Thakkar, Rakshit |
collection | PubMed |
description | A selective ultraperformance liquid chromatographic (UPLC) method for the determination of magnesium valproate and its process related impurities has been developed. The method includes reversed-phase Acquity BEH C(18) column with 100 mm × 2.1 mm i.d. and 1.7 µ particle size. The mobile phase consists of acetonitrile and 5 mM ammonium dihydrogen orthophosphate with pH = 3.0 at 45 : 55 isocratic elution. The flow rate was set at 0.3 mL/min and UV detection was performed at 215 nm. A system suitability test (SST) was developed to govern the quality of the separation. The developed method has been validated further with respect to linearity, accuracy, precision, selectivity, LOD, LOQ, and robustness. Different batches of samples were examined using this method; the method proved to be successful when applied to analyze a marketed magnesium valproate formulation. |
format | Online Article Text |
id | pubmed-4897345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48973452016-06-28 Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography Thakkar, Rakshit Saravaia, Hitesh Patel, Madhavi Shah, Anamik Int Sch Res Notices Research Article A selective ultraperformance liquid chromatographic (UPLC) method for the determination of magnesium valproate and its process related impurities has been developed. The method includes reversed-phase Acquity BEH C(18) column with 100 mm × 2.1 mm i.d. and 1.7 µ particle size. The mobile phase consists of acetonitrile and 5 mM ammonium dihydrogen orthophosphate with pH = 3.0 at 45 : 55 isocratic elution. The flow rate was set at 0.3 mL/min and UV detection was performed at 215 nm. A system suitability test (SST) was developed to govern the quality of the separation. The developed method has been validated further with respect to linearity, accuracy, precision, selectivity, LOD, LOQ, and robustness. Different batches of samples were examined using this method; the method proved to be successful when applied to analyze a marketed magnesium valproate formulation. Hindawi Publishing Corporation 2014-07-14 /pmc/articles/PMC4897345/ /pubmed/27355082 http://dx.doi.org/10.1155/2014/412704 Text en Copyright © 2014 Rakshit Thakkar et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Thakkar, Rakshit Saravaia, Hitesh Patel, Madhavi Shah, Anamik Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography |
title | Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography |
title_full | Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography |
title_fullStr | Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography |
title_full_unstemmed | Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography |
title_short | Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography |
title_sort | determination of magnesium valproate and its process related impurities by ultraperformance liquid chromatography |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897345/ https://www.ncbi.nlm.nih.gov/pubmed/27355082 http://dx.doi.org/10.1155/2014/412704 |
work_keys_str_mv | AT thakkarrakshit determinationofmagnesiumvalproateanditsprocessrelatedimpuritiesbyultraperformanceliquidchromatography AT saravaiahitesh determinationofmagnesiumvalproateanditsprocessrelatedimpuritiesbyultraperformanceliquidchromatography AT patelmadhavi determinationofmagnesiumvalproateanditsprocessrelatedimpuritiesbyultraperformanceliquidchromatography AT shahanamik determinationofmagnesiumvalproateanditsprocessrelatedimpuritiesbyultraperformanceliquidchromatography |