Cargando…

In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960

Background. Tuberculosis (TB) is a chronic bacterial disease. Mycobacterium tuberculosis, being the leading member of the MTB complex, is the main cause of tuberculosis worldwide. Tuberculosis is managed with combination of drugs: streptomycin, isoniazid, rifampicin, ethambutol, and pyrazinamide. Ov...

Descripción completa

Detalles Bibliográficos
Autores principales: Hannan, Abdul, Munir, Saira, Arshad, Muhammad Usman, Bashir, Nabila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897373/
https://www.ncbi.nlm.nih.gov/pubmed/27379286
http://dx.doi.org/10.1155/2014/490589
_version_ 1782436145076895744
author Hannan, Abdul
Munir, Saira
Arshad, Muhammad Usman
Bashir, Nabila
author_facet Hannan, Abdul
Munir, Saira
Arshad, Muhammad Usman
Bashir, Nabila
author_sort Hannan, Abdul
collection PubMed
description Background. Tuberculosis (TB) is a chronic bacterial disease. Mycobacterium tuberculosis, being the leading member of the MTB complex, is the main cause of tuberculosis worldwide. Tuberculosis is managed with combination of drugs: streptomycin, isoniazid, rifampicin, ethambutol, and pyrazinamide. Over the recent past years resistance against first line antituberculous drugs has emerged rapidly throughout the world resulting in MDR strains. The new threat in the management of MDR-TB is the development of resistance against second line drugs: aminoglycosides, polypeptides, fluoroquinolones, and thioamides. Multidrug resistant (MDR) and extensively drug resistant TB (XDR) strains have become a major concern to control TB particularly in the developing countries. The need of the hour is to look for new modalities having antimycobacterial activity. Honey has been well known for its antibacterial activity. We intended to explore its antimycobacterial activity against MDR-TB. Objective. The objective of this study was to determine whether Pakistani Beri honey has any antimycobacterial activity. Method. The study was conducted in the Department of Microbiology, University of Health Sciences, Lahore. Clinical isolates (n = 21) of MDR-MTB were evaluated for their susceptibility to Beri honey. The isolates were provided, courtesy of Pakistan Medical Research Council. These isolates were identified by MTBc ID test (Becton & Dickinson) and further tested for their antimycobacterial activity using Beri honey. The honey was tested at the following concentrations (v/v): 1%, 2%, 3%, 4%, and 5% in MGIT 960. Growth controls were also inoculated with each isolate (growth control has no concentration of honey, only containing growth of isolate). Results. MDR-TB isolates (n = 21) were tested; 3 (14%) isolates were susceptible at 1% v/v honey, while at 2% v/v of honey 18 (86%) isolates were found to be susceptible. All the 21 isolates (n = 21) were susceptible at 3% v/v of honey. Conclusion. The present study clearly demonstrates that Pakistani Beri honey possesses significant antimycobacterial activity in vitro. The antimycobacterial activity of Pakistani Beri honey may, therefore, be exploited in an appropriate mouse model.
format Online
Article
Text
id pubmed-4897373
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-48973732016-07-04 In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960 Hannan, Abdul Munir, Saira Arshad, Muhammad Usman Bashir, Nabila Int Sch Res Notices Research Article Background. Tuberculosis (TB) is a chronic bacterial disease. Mycobacterium tuberculosis, being the leading member of the MTB complex, is the main cause of tuberculosis worldwide. Tuberculosis is managed with combination of drugs: streptomycin, isoniazid, rifampicin, ethambutol, and pyrazinamide. Over the recent past years resistance against first line antituberculous drugs has emerged rapidly throughout the world resulting in MDR strains. The new threat in the management of MDR-TB is the development of resistance against second line drugs: aminoglycosides, polypeptides, fluoroquinolones, and thioamides. Multidrug resistant (MDR) and extensively drug resistant TB (XDR) strains have become a major concern to control TB particularly in the developing countries. The need of the hour is to look for new modalities having antimycobacterial activity. Honey has been well known for its antibacterial activity. We intended to explore its antimycobacterial activity against MDR-TB. Objective. The objective of this study was to determine whether Pakistani Beri honey has any antimycobacterial activity. Method. The study was conducted in the Department of Microbiology, University of Health Sciences, Lahore. Clinical isolates (n = 21) of MDR-MTB were evaluated for their susceptibility to Beri honey. The isolates were provided, courtesy of Pakistan Medical Research Council. These isolates were identified by MTBc ID test (Becton & Dickinson) and further tested for their antimycobacterial activity using Beri honey. The honey was tested at the following concentrations (v/v): 1%, 2%, 3%, 4%, and 5% in MGIT 960. Growth controls were also inoculated with each isolate (growth control has no concentration of honey, only containing growth of isolate). Results. MDR-TB isolates (n = 21) were tested; 3 (14%) isolates were susceptible at 1% v/v honey, while at 2% v/v of honey 18 (86%) isolates were found to be susceptible. All the 21 isolates (n = 21) were susceptible at 3% v/v of honey. Conclusion. The present study clearly demonstrates that Pakistani Beri honey possesses significant antimycobacterial activity in vitro. The antimycobacterial activity of Pakistani Beri honey may, therefore, be exploited in an appropriate mouse model. Hindawi Publishing Corporation 2014-10-07 /pmc/articles/PMC4897373/ /pubmed/27379286 http://dx.doi.org/10.1155/2014/490589 Text en Copyright © 2014 Abdul Hannan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hannan, Abdul
Munir, Saira
Arshad, Muhammad Usman
Bashir, Nabila
In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960
title In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960
title_full In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960
title_fullStr In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960
title_full_unstemmed In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960
title_short In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960
title_sort in vitro antimycobacterial activity of pakistani beri honey using bactec mgit 960
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897373/
https://www.ncbi.nlm.nih.gov/pubmed/27379286
http://dx.doi.org/10.1155/2014/490589
work_keys_str_mv AT hannanabdul invitroantimycobacterialactivityofpakistaniberihoneyusingbactecmgit960
AT munirsaira invitroantimycobacterialactivityofpakistaniberihoneyusingbactecmgit960
AT arshadmuhammadusman invitroantimycobacterialactivityofpakistaniberihoneyusingbactecmgit960
AT bashirnabila invitroantimycobacterialactivityofpakistaniberihoneyusingbactecmgit960