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STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells

Stathmin 1 (STMN1) is a biomarker in several types of neoplasms. It plays an important role in cell cycle progression, mitosis, signal transduction and cell migration. In ovaries, STMN1 is predominantly expressed in granulosa cells (GCs). However, little is known about the role of STMN1 in ovary. In...

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Autores principales: Dou, Yun-De, Zhao, Han, Huang, Tao, Zhao, Shi-Gang, Liu, Xiao-Man, Yu, Xiao-Chen, Ma, Zeng-Xiang, Zhang, Yu-Chao, Liu, Tao, Gao, Xuan, Li, Lei, Lu, Gang, Chan, Wai-Yee, Gao, Fei, Liu, Hong-Bin, Chen, Zi-Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897624/
https://www.ncbi.nlm.nih.gov/pubmed/27270953
http://dx.doi.org/10.1038/srep26691
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author Dou, Yun-De
Zhao, Han
Huang, Tao
Zhao, Shi-Gang
Liu, Xiao-Man
Yu, Xiao-Chen
Ma, Zeng-Xiang
Zhang, Yu-Chao
Liu, Tao
Gao, Xuan
Li, Lei
Lu, Gang
Chan, Wai-Yee
Gao, Fei
Liu, Hong-Bin
Chen, Zi-Jiang
author_facet Dou, Yun-De
Zhao, Han
Huang, Tao
Zhao, Shi-Gang
Liu, Xiao-Man
Yu, Xiao-Chen
Ma, Zeng-Xiang
Zhang, Yu-Chao
Liu, Tao
Gao, Xuan
Li, Lei
Lu, Gang
Chan, Wai-Yee
Gao, Fei
Liu, Hong-Bin
Chen, Zi-Jiang
author_sort Dou, Yun-De
collection PubMed
description Stathmin 1 (STMN1) is a biomarker in several types of neoplasms. It plays an important role in cell cycle progression, mitosis, signal transduction and cell migration. In ovaries, STMN1 is predominantly expressed in granulosa cells (GCs). However, little is known about the role of STMN1 in ovary. In this study, we demonstrated that STMN1 is overexpressed in GCs in patients with polycystic ovary syndrome (PCOS). In mouse primary GCs, the overexpression of STMN1 stimulated progesterone production, whereas knockdown of STMN1 decreased progesterone production. We also found that STMN1 positively regulates the expression of Star (steroidogenic acute regulatory protein) and Cyp11a1 (cytochrome P450 family 11 subfamily A member 1). Promoter and ChIP assays indicated that STMN1 increased the transcriptional activity of Star and Cyp11a1 by binding to their promoter regions. The data suggest that STMN1 mediates the progesterone production by modulating the promoter activity of Star and Cyp11a1. Together, our findings provide novel insights into the molecular mechanisms of STMN1 in ovary GC steroidogenesis. A better understanding of this potential interaction between STMN1 and Star in progesterone biosynthesis in GCs will facilitate the discovery of new therapeutic targets in PCOS.
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spelling pubmed-48976242016-06-10 STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells Dou, Yun-De Zhao, Han Huang, Tao Zhao, Shi-Gang Liu, Xiao-Man Yu, Xiao-Chen Ma, Zeng-Xiang Zhang, Yu-Chao Liu, Tao Gao, Xuan Li, Lei Lu, Gang Chan, Wai-Yee Gao, Fei Liu, Hong-Bin Chen, Zi-Jiang Sci Rep Article Stathmin 1 (STMN1) is a biomarker in several types of neoplasms. It plays an important role in cell cycle progression, mitosis, signal transduction and cell migration. In ovaries, STMN1 is predominantly expressed in granulosa cells (GCs). However, little is known about the role of STMN1 in ovary. In this study, we demonstrated that STMN1 is overexpressed in GCs in patients with polycystic ovary syndrome (PCOS). In mouse primary GCs, the overexpression of STMN1 stimulated progesterone production, whereas knockdown of STMN1 decreased progesterone production. We also found that STMN1 positively regulates the expression of Star (steroidogenic acute regulatory protein) and Cyp11a1 (cytochrome P450 family 11 subfamily A member 1). Promoter and ChIP assays indicated that STMN1 increased the transcriptional activity of Star and Cyp11a1 by binding to their promoter regions. The data suggest that STMN1 mediates the progesterone production by modulating the promoter activity of Star and Cyp11a1. Together, our findings provide novel insights into the molecular mechanisms of STMN1 in ovary GC steroidogenesis. A better understanding of this potential interaction between STMN1 and Star in progesterone biosynthesis in GCs will facilitate the discovery of new therapeutic targets in PCOS. Nature Publishing Group 2016-06-08 /pmc/articles/PMC4897624/ /pubmed/27270953 http://dx.doi.org/10.1038/srep26691 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Dou, Yun-De
Zhao, Han
Huang, Tao
Zhao, Shi-Gang
Liu, Xiao-Man
Yu, Xiao-Chen
Ma, Zeng-Xiang
Zhang, Yu-Chao
Liu, Tao
Gao, Xuan
Li, Lei
Lu, Gang
Chan, Wai-Yee
Gao, Fei
Liu, Hong-Bin
Chen, Zi-Jiang
STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
title STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
title_full STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
title_fullStr STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
title_full_unstemmed STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
title_short STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
title_sort stmn1 promotes progesterone production via star up-regulation in mouse granulosa cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897624/
https://www.ncbi.nlm.nih.gov/pubmed/27270953
http://dx.doi.org/10.1038/srep26691
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