Cargando…

Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well

Coal bed methane (CBM) is generated primarily through the microbial degradation of coal. Despite a limited understanding of the microorganisms responsible for this process, there is significant interest in developing methods to stimulate additional methane production from CBM wells. Physical techniq...

Descripción completa

Detalles Bibliográficos
Autores principales: Robbins, Steven J., Evans, Paul N., Parks, Donovan H., Golding, Suzanne D., Tyson, Gene W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897734/
https://www.ncbi.nlm.nih.gov/pubmed/27375557
http://dx.doi.org/10.3389/fmicb.2016.00731
_version_ 1782436225317076992
author Robbins, Steven J.
Evans, Paul N.
Parks, Donovan H.
Golding, Suzanne D.
Tyson, Gene W.
author_facet Robbins, Steven J.
Evans, Paul N.
Parks, Donovan H.
Golding, Suzanne D.
Tyson, Gene W.
author_sort Robbins, Steven J.
collection PubMed
description Coal bed methane (CBM) is generated primarily through the microbial degradation of coal. Despite a limited understanding of the microorganisms responsible for this process, there is significant interest in developing methods to stimulate additional methane production from CBM wells. Physical techniques including hydraulic fracture stimulation are commonly applied to CBM wells, however the effects of specific additives contained in hydraulic fracture fluids on native CBM microbial communities are poorly understood. Here, metagenomic sequencing was applied to the formation waters of a hydraulically fractured and several non-fractured CBM production wells to determine the effect of this stimulation technique on the in-situ microbial community. The hydraulically fractured well was dominated by two microbial populations belonging to the class Phycisphaerae (within phylum Planctomycetes) and candidate phylum Aminicenantes. Populations from these phyla were absent or present at extremely low abundance in non-fractured CBM wells. Detailed metabolic reconstruction of near-complete genomes from these populations showed that their high relative abundance in the hydraulically fractured CBM well could be explained by the introduction of additional carbon sources, electron acceptors, and biocides contained in the hydraulic fracture fluid.
format Online
Article
Text
id pubmed-4897734
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-48977342016-07-01 Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well Robbins, Steven J. Evans, Paul N. Parks, Donovan H. Golding, Suzanne D. Tyson, Gene W. Front Microbiol Microbiology Coal bed methane (CBM) is generated primarily through the microbial degradation of coal. Despite a limited understanding of the microorganisms responsible for this process, there is significant interest in developing methods to stimulate additional methane production from CBM wells. Physical techniques including hydraulic fracture stimulation are commonly applied to CBM wells, however the effects of specific additives contained in hydraulic fracture fluids on native CBM microbial communities are poorly understood. Here, metagenomic sequencing was applied to the formation waters of a hydraulically fractured and several non-fractured CBM production wells to determine the effect of this stimulation technique on the in-situ microbial community. The hydraulically fractured well was dominated by two microbial populations belonging to the class Phycisphaerae (within phylum Planctomycetes) and candidate phylum Aminicenantes. Populations from these phyla were absent or present at extremely low abundance in non-fractured CBM wells. Detailed metabolic reconstruction of near-complete genomes from these populations showed that their high relative abundance in the hydraulically fractured CBM well could be explained by the introduction of additional carbon sources, electron acceptors, and biocides contained in the hydraulic fracture fluid. Frontiers Media S.A. 2016-06-08 /pmc/articles/PMC4897734/ /pubmed/27375557 http://dx.doi.org/10.3389/fmicb.2016.00731 Text en Copyright © 2016 Robbins, Evans, Parks, Golding and Tyson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Robbins, Steven J.
Evans, Paul N.
Parks, Donovan H.
Golding, Suzanne D.
Tyson, Gene W.
Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well
title Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well
title_full Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well
title_fullStr Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well
title_full_unstemmed Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well
title_short Genome-Centric Analysis of Microbial Populations Enriched by Hydraulic Fracture Fluid Additives in a Coal Bed Methane Production Well
title_sort genome-centric analysis of microbial populations enriched by hydraulic fracture fluid additives in a coal bed methane production well
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897734/
https://www.ncbi.nlm.nih.gov/pubmed/27375557
http://dx.doi.org/10.3389/fmicb.2016.00731
work_keys_str_mv AT robbinsstevenj genomecentricanalysisofmicrobialpopulationsenrichedbyhydraulicfracturefluidadditivesinacoalbedmethaneproductionwell
AT evanspauln genomecentricanalysisofmicrobialpopulationsenrichedbyhydraulicfracturefluidadditivesinacoalbedmethaneproductionwell
AT parksdonovanh genomecentricanalysisofmicrobialpopulationsenrichedbyhydraulicfracturefluidadditivesinacoalbedmethaneproductionwell
AT goldingsuzanned genomecentricanalysisofmicrobialpopulationsenrichedbyhydraulicfracturefluidadditivesinacoalbedmethaneproductionwell
AT tysongenew genomecentricanalysisofmicrobialpopulationsenrichedbyhydraulicfracturefluidadditivesinacoalbedmethaneproductionwell