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RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation
BACKGROUND: In order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. This could be achieved through an optimization of both root architecture and root...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897935/ https://www.ncbi.nlm.nih.gov/pubmed/27279895 http://dx.doi.org/10.1186/s13007-016-0131-9 |
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author | Jeudy, Christian Adrian, Marielle Baussard, Christophe Bernard, Céline Bernaud, Eric Bourion, Virginie Busset, Hughes Cabrera-Bosquet, Llorenç Cointault, Frédéric Han, Simeng Lamboeuf, Mickael Moreau, Delphine Pivato, Barbara Prudent, Marion Trouvelot, Sophie Truong, Hoai Nam Vernoud, Vanessa Voisin, Anne-Sophie Wipf, Daniel Salon, Christophe |
author_facet | Jeudy, Christian Adrian, Marielle Baussard, Christophe Bernard, Céline Bernaud, Eric Bourion, Virginie Busset, Hughes Cabrera-Bosquet, Llorenç Cointault, Frédéric Han, Simeng Lamboeuf, Mickael Moreau, Delphine Pivato, Barbara Prudent, Marion Trouvelot, Sophie Truong, Hoai Nam Vernoud, Vanessa Voisin, Anne-Sophie Wipf, Daniel Salon, Christophe |
author_sort | Jeudy, Christian |
collection | PubMed |
description | BACKGROUND: In order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. This could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. The development of devices suitable for high-throughput phenotyping of root structures remains a major bottleneck. RESULTS: Rhizotrons suitable for plant growth in controlled conditions and non-invasive image acquisition of plant shoot and root systems (RhizoTubes) are described. These RhizoTubes allow growing one to six plants simultaneously, having a maximum height of 1.1 m, up to 8 weeks, depending on plant species. Both shoot and root compartment can be imaged automatically and non-destructively throughout the experiment thanks to an imaging cabin (RhizoCab). RhizoCab contains robots and imaging equipment for obtaining high-resolution pictures of plant roots. Using this versatile experimental setup, we illustrate how some morphometric root traits can be determined for various species including model (Medicago truncatula), crops (Pisum sativum, Brassica napus, Vitis vinifera, Triticum aestivum) and weed (Vulpia myuros) species grown under non-limiting conditions or submitted to various abiotic and biotic constraints. The measurement of the root phenotypic traits using this system was compared to that obtained using “classic” growth conditions in pots. CONCLUSIONS: This integrated system, to include 1200 Rhizotubes, will allow high-throughput phenotyping of plant shoots and roots under various abiotic and biotic environmental conditions. Our system allows an easy visualization or extraction of roots and measurement of root traits for high-throughput or kinetic analyses. The utility of this system for studying root system architecture will greatly facilitate the identification of genetic and environmental determinants of key root traits involved in crop responses to stresses, including interactions with soil microorganisms. |
format | Online Article Text |
id | pubmed-4897935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48979352016-06-09 RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation Jeudy, Christian Adrian, Marielle Baussard, Christophe Bernard, Céline Bernaud, Eric Bourion, Virginie Busset, Hughes Cabrera-Bosquet, Llorenç Cointault, Frédéric Han, Simeng Lamboeuf, Mickael Moreau, Delphine Pivato, Barbara Prudent, Marion Trouvelot, Sophie Truong, Hoai Nam Vernoud, Vanessa Voisin, Anne-Sophie Wipf, Daniel Salon, Christophe Plant Methods Methodology BACKGROUND: In order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. This could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. The development of devices suitable for high-throughput phenotyping of root structures remains a major bottleneck. RESULTS: Rhizotrons suitable for plant growth in controlled conditions and non-invasive image acquisition of plant shoot and root systems (RhizoTubes) are described. These RhizoTubes allow growing one to six plants simultaneously, having a maximum height of 1.1 m, up to 8 weeks, depending on plant species. Both shoot and root compartment can be imaged automatically and non-destructively throughout the experiment thanks to an imaging cabin (RhizoCab). RhizoCab contains robots and imaging equipment for obtaining high-resolution pictures of plant roots. Using this versatile experimental setup, we illustrate how some morphometric root traits can be determined for various species including model (Medicago truncatula), crops (Pisum sativum, Brassica napus, Vitis vinifera, Triticum aestivum) and weed (Vulpia myuros) species grown under non-limiting conditions or submitted to various abiotic and biotic constraints. The measurement of the root phenotypic traits using this system was compared to that obtained using “classic” growth conditions in pots. CONCLUSIONS: This integrated system, to include 1200 Rhizotubes, will allow high-throughput phenotyping of plant shoots and roots under various abiotic and biotic environmental conditions. Our system allows an easy visualization or extraction of roots and measurement of root traits for high-throughput or kinetic analyses. The utility of this system for studying root system architecture will greatly facilitate the identification of genetic and environmental determinants of key root traits involved in crop responses to stresses, including interactions with soil microorganisms. BioMed Central 2016-06-07 /pmc/articles/PMC4897935/ /pubmed/27279895 http://dx.doi.org/10.1186/s13007-016-0131-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Jeudy, Christian Adrian, Marielle Baussard, Christophe Bernard, Céline Bernaud, Eric Bourion, Virginie Busset, Hughes Cabrera-Bosquet, Llorenç Cointault, Frédéric Han, Simeng Lamboeuf, Mickael Moreau, Delphine Pivato, Barbara Prudent, Marion Trouvelot, Sophie Truong, Hoai Nam Vernoud, Vanessa Voisin, Anne-Sophie Wipf, Daniel Salon, Christophe RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
title | RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
title_full | RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
title_fullStr | RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
title_full_unstemmed | RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
title_short | RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
title_sort | rhizotubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897935/ https://www.ncbi.nlm.nih.gov/pubmed/27279895 http://dx.doi.org/10.1186/s13007-016-0131-9 |
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