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Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

BACKGROUND: Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buf...

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Detalles Bibliográficos
Autores principales:	Upadhyay, Vaibhav, Singh, Anupam, Jha, Divya, Singh, Akansha, Panda, Amulya K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4898390/ https://www.ncbi.nlm.nih.gov/pubmed/27277580 http://dx.doi.org/10.1186/s12934-016-0504-9

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