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Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance

Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram-negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene (mcr-1) is prevalent in food animals and human beings worldwide. Mor...

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Autores principales: Zhang, Huimin, Seward, Christopher H., Wu, Zuowei, Ye, Huiyan, Feng, Youjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Science China Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899488/
https://www.ncbi.nlm.nih.gov/pubmed/27358749
http://dx.doi.org/10.1007/s11434-016-1086-y
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author Zhang, Huimin
Seward, Christopher H.
Wu, Zuowei
Ye, Huiyan
Feng, Youjun
author_facet Zhang, Huimin
Seward, Christopher H.
Wu, Zuowei
Ye, Huiyan
Feng, Youjun
author_sort Zhang, Huimin
collection PubMed
description Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram-negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene (mcr-1) is prevalent in food animals and human beings worldwide. More importantly, the mcr-1 gene was found to be co-localized with other antibiotic resistance genes, raising the possibility that super-bugs with pan-drug resistance are emerging. However, little is reported on the genomes of the mcr-1-positive bacterial host reservoirs. Here we report genome sequencing of three human isolates of the mcr-1-positive Escherichia coli (E15004, E15015 and E15017) and define general features through analyses of bacterial comparative genomics. Further genomic mining together with sequence typing allowed us to elucidate that the MCR-1-carrying E. coli E15017 belongs to the sequence type ST648 and coproduces extended-spectrum β-lactamase (ESBL). Given the fact that ST648 has been known to associate with either New Delhi metallo-β-lactamase 1 or ESBL, our results highlighted the possibility of ST648 as an epidemic clone with multidrug resistances. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11434-016-1086-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-48994882016-06-27 Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance Zhang, Huimin Seward, Christopher H. Wu, Zuowei Ye, Huiyan Feng, Youjun Sci Bull (Beijing) Letter Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram-negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene (mcr-1) is prevalent in food animals and human beings worldwide. More importantly, the mcr-1 gene was found to be co-localized with other antibiotic resistance genes, raising the possibility that super-bugs with pan-drug resistance are emerging. However, little is reported on the genomes of the mcr-1-positive bacterial host reservoirs. Here we report genome sequencing of three human isolates of the mcr-1-positive Escherichia coli (E15004, E15015 and E15017) and define general features through analyses of bacterial comparative genomics. Further genomic mining together with sequence typing allowed us to elucidate that the MCR-1-carrying E. coli E15017 belongs to the sequence type ST648 and coproduces extended-spectrum β-lactamase (ESBL). Given the fact that ST648 has been known to associate with either New Delhi metallo-β-lactamase 1 or ESBL, our results highlighted the possibility of ST648 as an epidemic clone with multidrug resistances. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11434-016-1086-y) contains supplementary material, which is available to authorized users. Science China Press 2016-05-19 2016 /pmc/articles/PMC4899488/ /pubmed/27358749 http://dx.doi.org/10.1007/s11434-016-1086-y Text en © Science China Press and Springer-Verlag Berlin Heidelberg 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Letter
Zhang, Huimin
Seward, Christopher H.
Wu, Zuowei
Ye, Huiyan
Feng, Youjun
Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance
title Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance
title_full Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance
title_fullStr Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance
title_full_unstemmed Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance
title_short Genomic insights into the ESBL and MCR-1-producing ST648 Escherichiacoli with multi-drug resistance
title_sort genomic insights into the esbl and mcr-1-producing st648 escherichiacoli with multi-drug resistance
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899488/
https://www.ncbi.nlm.nih.gov/pubmed/27358749
http://dx.doi.org/10.1007/s11434-016-1086-y
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