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Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59

Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Mole...

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Autores principales: Heider, Susanne, Kleinberger, Sandra, Kochan, Feliks, Dangerfield, John A., Metzner, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500/
https://www.ncbi.nlm.nih.gov/pubmed/27170144
http://dx.doi.org/10.1007/s12033-016-9944-z
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author Heider, Susanne
Kleinberger, Sandra
Kochan, Feliks
Dangerfield, John A.
Metzner, Christoph
author_facet Heider, Susanne
Kleinberger, Sandra
Kochan, Feliks
Dangerfield, John A.
Metzner, Christoph
author_sort Heider, Susanne
collection PubMed
description Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12033-016-9944-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-48995002016-06-27 Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph Mol Biotechnol Original Paper Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12033-016-9944-z) contains supplementary material, which is available to authorized users. Springer US 2016-05-11 2016 /pmc/articles/PMC4899500/ /pubmed/27170144 http://dx.doi.org/10.1007/s12033-016-9944-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Heider, Susanne
Kleinberger, Sandra
Kochan, Feliks
Dangerfield, John A.
Metzner, Christoph
Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_full Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_fullStr Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_full_unstemmed Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_short Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_sort immune protection of retroviral vectors upon molecular painting with the complement regulatory protein cd59
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500/
https://www.ncbi.nlm.nih.gov/pubmed/27170144
http://dx.doi.org/10.1007/s12033-016-9944-z
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