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Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Mole...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500/ https://www.ncbi.nlm.nih.gov/pubmed/27170144 http://dx.doi.org/10.1007/s12033-016-9944-z |
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author | Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph |
author_facet | Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph |
author_sort | Heider, Susanne |
collection | PubMed |
description | Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12033-016-9944-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4899500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-48995002016-06-27 Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph Mol Biotechnol Original Paper Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12033-016-9944-z) contains supplementary material, which is available to authorized users. Springer US 2016-05-11 2016 /pmc/articles/PMC4899500/ /pubmed/27170144 http://dx.doi.org/10.1007/s12033-016-9944-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 |
title | Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 |
title_full | Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 |
title_fullStr | Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 |
title_full_unstemmed | Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 |
title_short | Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 |
title_sort | immune protection of retroviral vectors upon molecular painting with the complement regulatory protein cd59 |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500/ https://www.ncbi.nlm.nih.gov/pubmed/27170144 http://dx.doi.org/10.1007/s12033-016-9944-z |
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