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Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green
Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899706/ https://www.ncbi.nlm.nih.gov/pubmed/27277343 http://dx.doi.org/10.1038/srep27525 |
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| author | Shinden, Yoshiaki Ueo, Hiroki Tobo, Taro Gamachi, Ayako Utou, Mitsuaki Komatsu, Hisateru Nambara, Sho Saito, Tomoko Ueda, Masami Hirata, Hidenari Sakimura, Shotaro Takano, Yuki Uchi, Ryutaro Kurashige, Junji Akiyoshi, Sayuri Iguchi, Tomohiro Eguchi, Hidetoshi Sugimachi, Keishi Kubota, Yoko Kai, Yuichiro Shibuta, Kenji Kijima, Yuko Yoshinaka, Heiji Natsugoe, Shoji Mori, Masaki Maehara, Yoshihiko Sakabe, Masayo Kamiya, Mako Kakareka, John W. Pohida, Thomas J. Choyke, Peter L. Kobayashi, Hisataka Ueo, Hiroaki Urano, Yasuteru Mimori, Koshi |
| author_facet | Shinden, Yoshiaki Ueo, Hiroki Tobo, Taro Gamachi, Ayako Utou, Mitsuaki Komatsu, Hisateru Nambara, Sho Saito, Tomoko Ueda, Masami Hirata, Hidenari Sakimura, Shotaro Takano, Yuki Uchi, Ryutaro Kurashige, Junji Akiyoshi, Sayuri Iguchi, Tomohiro Eguchi, Hidetoshi Sugimachi, Keishi Kubota, Yoko Kai, Yuichiro Shibuta, Kenji Kijima, Yuko Yoshinaka, Heiji Natsugoe, Shoji Mori, Masaki Maehara, Yoshihiko Sakabe, Masayo Kamiya, Mako Kakareka, John W. Pohida, Thomas J. Choyke, Peter L. Kobayashi, Hisataka Ueo, Hiroaki Urano, Yasuteru Mimori, Koshi |
| author_sort | Shinden, Yoshiaki |
| collection | PubMed |
| description | Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists. |
| format | Online Article Text |
| id | pubmed-4899706 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-48997062016-06-13 Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green Shinden, Yoshiaki Ueo, Hiroki Tobo, Taro Gamachi, Ayako Utou, Mitsuaki Komatsu, Hisateru Nambara, Sho Saito, Tomoko Ueda, Masami Hirata, Hidenari Sakimura, Shotaro Takano, Yuki Uchi, Ryutaro Kurashige, Junji Akiyoshi, Sayuri Iguchi, Tomohiro Eguchi, Hidetoshi Sugimachi, Keishi Kubota, Yoko Kai, Yuichiro Shibuta, Kenji Kijima, Yuko Yoshinaka, Heiji Natsugoe, Shoji Mori, Masaki Maehara, Yoshihiko Sakabe, Masayo Kamiya, Mako Kakareka, John W. Pohida, Thomas J. Choyke, Peter L. Kobayashi, Hisataka Ueo, Hiroaki Urano, Yasuteru Mimori, Koshi Sci Rep Article Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists. Nature Publishing Group 2016-06-09 /pmc/articles/PMC4899706/ /pubmed/27277343 http://dx.doi.org/10.1038/srep27525 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Article Shinden, Yoshiaki Ueo, Hiroki Tobo, Taro Gamachi, Ayako Utou, Mitsuaki Komatsu, Hisateru Nambara, Sho Saito, Tomoko Ueda, Masami Hirata, Hidenari Sakimura, Shotaro Takano, Yuki Uchi, Ryutaro Kurashige, Junji Akiyoshi, Sayuri Iguchi, Tomohiro Eguchi, Hidetoshi Sugimachi, Keishi Kubota, Yoko Kai, Yuichiro Shibuta, Kenji Kijima, Yuko Yoshinaka, Heiji Natsugoe, Shoji Mori, Masaki Maehara, Yoshihiko Sakabe, Masayo Kamiya, Mako Kakareka, John W. Pohida, Thomas J. Choyke, Peter L. Kobayashi, Hisataka Ueo, Hiroaki Urano, Yasuteru Mimori, Koshi Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| title | Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| title_full | Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| title_fullStr | Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| title_full_unstemmed | Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| title_short | Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| title_sort | rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899706/ https://www.ncbi.nlm.nih.gov/pubmed/27277343 http://dx.doi.org/10.1038/srep27525 |
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