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Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity

Marginal zone (MZ) B cells, representing a distinct subset of innate-like B cells, mount rapid T-independent responses to blood-borne antigens. They express low-affinity polyreactive antigen receptors that recognize both foreign and self-structures. The spleen is considered the exclusive site for mu...

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Autores principales: Palm, Anna-Karin E., Friedrich, Heike C., Kleinau, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899733/
https://www.ncbi.nlm.nih.gov/pubmed/27277419
http://dx.doi.org/10.1038/srep27687
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author Palm, Anna-Karin E.
Friedrich, Heike C.
Kleinau, Sandra
author_facet Palm, Anna-Karin E.
Friedrich, Heike C.
Kleinau, Sandra
author_sort Palm, Anna-Karin E.
collection PubMed
description Marginal zone (MZ) B cells, representing a distinct subset of innate-like B cells, mount rapid T-independent responses to blood-borne antigens. They express low-affinity polyreactive antigen receptors that recognize both foreign and self-structures. The spleen is considered the exclusive site for murine MZ B cells. However, we have here identified B cells with a MZ B-cell phenotype in the subcapsular sinuses of mouse lymph nodes. The nodal MZ (nMZ) B cells display high levels of IgM, costimulators and TLRs, and are represented by naïve and memory cells. The frequency of nMZ B cells is about 1–6% of nodal B cells depending on mouse strain, with higher numbers in older mice and a trend of increased numbers in females. There is a significant expansion of nMZ B cells following immunization with an autoantigen, but not after likewise immunization with a control protein or with the adjuvant alone. The nMZ B cells secrete autoantibodies upon activation and can efficiently present autoantigen to cognate T cells in vitro, inducing T-cell proliferation. The existence of self-reactive MZ B cells in lymph nodes may be a source of autoantigen-presenting cells that in an unfortunate environment may activate T cells leading to autoimmunity.
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spelling pubmed-48997332016-06-13 Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity Palm, Anna-Karin E. Friedrich, Heike C. Kleinau, Sandra Sci Rep Article Marginal zone (MZ) B cells, representing a distinct subset of innate-like B cells, mount rapid T-independent responses to blood-borne antigens. They express low-affinity polyreactive antigen receptors that recognize both foreign and self-structures. The spleen is considered the exclusive site for murine MZ B cells. However, we have here identified B cells with a MZ B-cell phenotype in the subcapsular sinuses of mouse lymph nodes. The nodal MZ (nMZ) B cells display high levels of IgM, costimulators and TLRs, and are represented by naïve and memory cells. The frequency of nMZ B cells is about 1–6% of nodal B cells depending on mouse strain, with higher numbers in older mice and a trend of increased numbers in females. There is a significant expansion of nMZ B cells following immunization with an autoantigen, but not after likewise immunization with a control protein or with the adjuvant alone. The nMZ B cells secrete autoantibodies upon activation and can efficiently present autoantigen to cognate T cells in vitro, inducing T-cell proliferation. The existence of self-reactive MZ B cells in lymph nodes may be a source of autoantigen-presenting cells that in an unfortunate environment may activate T cells leading to autoimmunity. Nature Publishing Group 2016-06-09 /pmc/articles/PMC4899733/ /pubmed/27277419 http://dx.doi.org/10.1038/srep27687 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Palm, Anna-Karin E.
Friedrich, Heike C.
Kleinau, Sandra
Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
title Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
title_full Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
title_fullStr Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
title_full_unstemmed Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
title_short Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
title_sort nodal marginal zone b cells in mice: a novel subset with dormant self-reactivity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899733/
https://www.ncbi.nlm.nih.gov/pubmed/27277419
http://dx.doi.org/10.1038/srep27687
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