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Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses

“Candidatus Liberibacter solanacearum” (Lso) has emerged as a serious threat world-wide. Five Lso haplotypes have been identified so far. Haplotypes A and B are present in the Americas and/or New Zealand, where they are vectored to solanaceous plants by the potato psyllid, Bactericera cockerelli (Šu...

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Autores principales: Yao, Jianxiu, Saenkham, Panatda, Levy, Julien, Ibanez, Freddy, Noroy, Christophe, Mendoza, Azucena, Huot, Ordom, Meyer, Damien F., Tamborindeguy, Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899927/
https://www.ncbi.nlm.nih.gov/pubmed/27376032
http://dx.doi.org/10.3389/fcimb.2016.00062
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author Yao, Jianxiu
Saenkham, Panatda
Levy, Julien
Ibanez, Freddy
Noroy, Christophe
Mendoza, Azucena
Huot, Ordom
Meyer, Damien F.
Tamborindeguy, Cecilia
author_facet Yao, Jianxiu
Saenkham, Panatda
Levy, Julien
Ibanez, Freddy
Noroy, Christophe
Mendoza, Azucena
Huot, Ordom
Meyer, Damien F.
Tamborindeguy, Cecilia
author_sort Yao, Jianxiu
collection PubMed
description “Candidatus Liberibacter solanacearum” (Lso) has emerged as a serious threat world-wide. Five Lso haplotypes have been identified so far. Haplotypes A and B are present in the Americas and/or New Zealand, where they are vectored to solanaceous plants by the potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae). The fastidious nature of these pathogens has hindered the study of the interactions with their eukaryotic hosts (vector and plant). To understand the strategies used by these pathogens to infect their vector, the effects of each Lso haplotype (A or B) on psyllid fitness was investigated, and genome-wide transcriptomic and RT-qPCR analyses were performed to evaluate Lso gene expression in association with its vector. Results showed that psyllids infected with haplotype B had significantly lower percentage of nymphal survival compared to psyllids infected with haplotype A. Although overall gene expression across Lso genome was similar between the two Lso haplotypes, differences in the expression of key candidate genes were found. Among the 16 putative type IV effector genes tested, four of them were differentially expressed between Lso haplotypes, while no differences in gene expression were measured by qPCR or transcriptomic analysis for the rest of the genes. This study provides new information regarding the pathogenesis of Lso haplotypes in their insect vector.
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spelling pubmed-48999272016-07-01 Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses Yao, Jianxiu Saenkham, Panatda Levy, Julien Ibanez, Freddy Noroy, Christophe Mendoza, Azucena Huot, Ordom Meyer, Damien F. Tamborindeguy, Cecilia Front Cell Infect Microbiol Microbiology “Candidatus Liberibacter solanacearum” (Lso) has emerged as a serious threat world-wide. Five Lso haplotypes have been identified so far. Haplotypes A and B are present in the Americas and/or New Zealand, where they are vectored to solanaceous plants by the potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae). The fastidious nature of these pathogens has hindered the study of the interactions with their eukaryotic hosts (vector and plant). To understand the strategies used by these pathogens to infect their vector, the effects of each Lso haplotype (A or B) on psyllid fitness was investigated, and genome-wide transcriptomic and RT-qPCR analyses were performed to evaluate Lso gene expression in association with its vector. Results showed that psyllids infected with haplotype B had significantly lower percentage of nymphal survival compared to psyllids infected with haplotype A. Although overall gene expression across Lso genome was similar between the two Lso haplotypes, differences in the expression of key candidate genes were found. Among the 16 putative type IV effector genes tested, four of them were differentially expressed between Lso haplotypes, while no differences in gene expression were measured by qPCR or transcriptomic analysis for the rest of the genes. This study provides new information regarding the pathogenesis of Lso haplotypes in their insect vector. Frontiers Media S.A. 2016-06-09 /pmc/articles/PMC4899927/ /pubmed/27376032 http://dx.doi.org/10.3389/fcimb.2016.00062 Text en Copyright © 2016 Yao, Saenkham, Levy, Ibanez, Noroy, Mendoza, Huot, Meyer and Tamborindeguy. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yao, Jianxiu
Saenkham, Panatda
Levy, Julien
Ibanez, Freddy
Noroy, Christophe
Mendoza, Azucena
Huot, Ordom
Meyer, Damien F.
Tamborindeguy, Cecilia
Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses
title Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses
title_full Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses
title_fullStr Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses
title_full_unstemmed Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses
title_short Interactions “Candidatus Liberibacter solanacearum”—Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses
title_sort interactions “candidatus liberibacter solanacearum”—bactericera cockerelli: haplotype effect on vector fitness and gene expression analyses
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899927/
https://www.ncbi.nlm.nih.gov/pubmed/27376032
http://dx.doi.org/10.3389/fcimb.2016.00062
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