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Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System

BACKGROUND: Bisphenol A (BPA) and nonylphenol (NP) have harmful effects on the endocrine system of humans and animals. AIM: We sought to investigate the effect of three doses of BPA and NP on the reproductive parameters of rats. MATERIALS AND METHODS: Adult Wistar male rats weighing 150–200 g were u...

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Autores principales: Kazemi, Sohrab, Feizi, Farideh, Aghapour, Fahimaeh, Joorsaraee, Gholam Ali, Moghadamnia, Ali Akbar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899961/
https://www.ncbi.nlm.nih.gov/pubmed/27298816
http://dx.doi.org/10.4103/1947-2714.183012
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author Kazemi, Sohrab
Feizi, Farideh
Aghapour, Fahimaeh
Joorsaraee, Gholam Ali
Moghadamnia, Ali Akbar
author_facet Kazemi, Sohrab
Feizi, Farideh
Aghapour, Fahimaeh
Joorsaraee, Gholam Ali
Moghadamnia, Ali Akbar
author_sort Kazemi, Sohrab
collection PubMed
description BACKGROUND: Bisphenol A (BPA) and nonylphenol (NP) have harmful effects on the endocrine system of humans and animals. AIM: We sought to investigate the effect of three doses of BPA and NP on the reproductive parameters of rats. MATERIALS AND METHODS: Adult Wistar male rats weighing 150–200 g were used for a consecutive 35-day study. BPA and NP were given as gavage in three doses (5, 25, and 125 μg/kg). At the end of the study, the rats were anesthetized and 2 ml blood sample was obtained from the auxiliary venous plexus for the assessment of sex hormone levels. The testes were removed and kept in 10% formalin for the histomorphometric and histopathologic analyses. RESULTS: BPA and NP significantly decreased the body weight of the animals compared to the controls (P < 0.05). The seminiferous tubule diameter and thickness of the seminiferous epithelium were significantly decreased in the groups receiving BPA and NP compared to the control (P < 0.05). The number of seminiferous tubules in every experimental group increased, except for the highest dose of NP (125 μg/kg), which showed a significant difference compared to the controls (P < 0.05). The number of spermatocytes and spermatogonia (54.97 ± 5.824, 35.78 ± 3.956, respectively) in the group receiving NP (125 μg/kg) was significantly decreased compared to the other groups. Serum levels of luteinizing hormone, Follicle stimulating hormone, and testosterone did not show any significant change compared to the controls. CONCLUSION: Based on the results, all three doses of BPA and NP significantly produced weight loss, as well as destruction of the testis tissue and impairment of the spermatogenesis.
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spelling pubmed-48999612016-06-13 Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System Kazemi, Sohrab Feizi, Farideh Aghapour, Fahimaeh Joorsaraee, Gholam Ali Moghadamnia, Ali Akbar N Am J Med Sci Original Article BACKGROUND: Bisphenol A (BPA) and nonylphenol (NP) have harmful effects on the endocrine system of humans and animals. AIM: We sought to investigate the effect of three doses of BPA and NP on the reproductive parameters of rats. MATERIALS AND METHODS: Adult Wistar male rats weighing 150–200 g were used for a consecutive 35-day study. BPA and NP were given as gavage in three doses (5, 25, and 125 μg/kg). At the end of the study, the rats were anesthetized and 2 ml blood sample was obtained from the auxiliary venous plexus for the assessment of sex hormone levels. The testes were removed and kept in 10% formalin for the histomorphometric and histopathologic analyses. RESULTS: BPA and NP significantly decreased the body weight of the animals compared to the controls (P < 0.05). The seminiferous tubule diameter and thickness of the seminiferous epithelium were significantly decreased in the groups receiving BPA and NP compared to the control (P < 0.05). The number of seminiferous tubules in every experimental group increased, except for the highest dose of NP (125 μg/kg), which showed a significant difference compared to the controls (P < 0.05). The number of spermatocytes and spermatogonia (54.97 ± 5.824, 35.78 ± 3.956, respectively) in the group receiving NP (125 μg/kg) was significantly decreased compared to the other groups. Serum levels of luteinizing hormone, Follicle stimulating hormone, and testosterone did not show any significant change compared to the controls. CONCLUSION: Based on the results, all three doses of BPA and NP significantly produced weight loss, as well as destruction of the testis tissue and impairment of the spermatogenesis. Medknow Publications & Media Pvt Ltd 2016-05 /pmc/articles/PMC4899961/ /pubmed/27298816 http://dx.doi.org/10.4103/1947-2714.183012 Text en Copyright: © North American Journal of Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kazemi, Sohrab
Feizi, Farideh
Aghapour, Fahimaeh
Joorsaraee, Gholam Ali
Moghadamnia, Ali Akbar
Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System
title Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System
title_full Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System
title_fullStr Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System
title_full_unstemmed Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System
title_short Histopathology and Histomorphometric Investigation of Bisphenol A and Nonylphenol on the Male Rat Reproductive System
title_sort histopathology and histomorphometric investigation of bisphenol a and nonylphenol on the male rat reproductive system
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899961/
https://www.ncbi.nlm.nih.gov/pubmed/27298816
http://dx.doi.org/10.4103/1947-2714.183012
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