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Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints

Osteoarthritis (OA) is one of most common skeletal disorders and can affect synovial joints such as knee and ankle joints. α5 integrin, a major fibronectin receptor, is expressed in articular cartilage and has been demonstrated to play roles in synovial joint development and in the regulation of cho...

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Autores principales: Candela, Maria Elena, Wang, Chao, Gunawardena, Aruni T., Zhang, Kairui, Cantley, Leslie, Yasuhara, Rika, Usami, Yu, Francois, Noelle, Iwamoto, Masahiro, van der Flier, Arjan, Zhang, Yejia, Qin, Ling, Han, Lin, Enomoto-Iwamoto, Motomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900574/
https://www.ncbi.nlm.nih.gov/pubmed/27280771
http://dx.doi.org/10.1371/journal.pone.0156783
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author Candela, Maria Elena
Wang, Chao
Gunawardena, Aruni T.
Zhang, Kairui
Cantley, Leslie
Yasuhara, Rika
Usami, Yu
Francois, Noelle
Iwamoto, Masahiro
van der Flier, Arjan
Zhang, Yejia
Qin, Ling
Han, Lin
Enomoto-Iwamoto, Motomi
author_facet Candela, Maria Elena
Wang, Chao
Gunawardena, Aruni T.
Zhang, Kairui
Cantley, Leslie
Yasuhara, Rika
Usami, Yu
Francois, Noelle
Iwamoto, Masahiro
van der Flier, Arjan
Zhang, Yejia
Qin, Ling
Han, Lin
Enomoto-Iwamoto, Motomi
author_sort Candela, Maria Elena
collection PubMed
description Osteoarthritis (OA) is one of most common skeletal disorders and can affect synovial joints such as knee and ankle joints. α5 integrin, a major fibronectin receptor, is expressed in articular cartilage and has been demonstrated to play roles in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. We hypothesized that α5 integrin signaling is involved in pathogenesis of OA. To test this, we generated compound mice that conditionally ablate α5 integrin in the synovial joints using the Gdf5Cre system. The compound mice were born normally and had an overall appearance similar to the control mice. However, when the mutant mice received the OA surgery, they showed stronger resistance to osteoarthritic changes than the control. Specifically the mutant knee joints presented lower levels of cartilage matrix and structure loss and synovial changes and showed stronger biomechanical properties than the control knee joints. These findings indicate that α5 integrin may not be essential for synovial joint development but play a causative role in induction of osteoarthritic changes.
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spelling pubmed-49005742016-06-24 Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints Candela, Maria Elena Wang, Chao Gunawardena, Aruni T. Zhang, Kairui Cantley, Leslie Yasuhara, Rika Usami, Yu Francois, Noelle Iwamoto, Masahiro van der Flier, Arjan Zhang, Yejia Qin, Ling Han, Lin Enomoto-Iwamoto, Motomi PLoS One Research Article Osteoarthritis (OA) is one of most common skeletal disorders and can affect synovial joints such as knee and ankle joints. α5 integrin, a major fibronectin receptor, is expressed in articular cartilage and has been demonstrated to play roles in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. We hypothesized that α5 integrin signaling is involved in pathogenesis of OA. To test this, we generated compound mice that conditionally ablate α5 integrin in the synovial joints using the Gdf5Cre system. The compound mice were born normally and had an overall appearance similar to the control mice. However, when the mutant mice received the OA surgery, they showed stronger resistance to osteoarthritic changes than the control. Specifically the mutant knee joints presented lower levels of cartilage matrix and structure loss and synovial changes and showed stronger biomechanical properties than the control knee joints. These findings indicate that α5 integrin may not be essential for synovial joint development but play a causative role in induction of osteoarthritic changes. Public Library of Science 2016-06-09 /pmc/articles/PMC4900574/ /pubmed/27280771 http://dx.doi.org/10.1371/journal.pone.0156783 Text en © 2016 Candela et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Candela, Maria Elena
Wang, Chao
Gunawardena, Aruni T.
Zhang, Kairui
Cantley, Leslie
Yasuhara, Rika
Usami, Yu
Francois, Noelle
Iwamoto, Masahiro
van der Flier, Arjan
Zhang, Yejia
Qin, Ling
Han, Lin
Enomoto-Iwamoto, Motomi
Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints
title Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints
title_full Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints
title_fullStr Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints
title_full_unstemmed Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints
title_short Alpha 5 Integrin Mediates Osteoarthritic Changes in Mouse Knee Joints
title_sort alpha 5 integrin mediates osteoarthritic changes in mouse knee joints
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900574/
https://www.ncbi.nlm.nih.gov/pubmed/27280771
http://dx.doi.org/10.1371/journal.pone.0156783
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