Cargando…

A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis

Great strides into understanding protein folding have been made since the seminal work of Anfinsen over 40 years ago, but progress in the study of membrane protein folding has lagged behind that of their water soluble counterparts. Researchers in these fields continue to turn to more advanced techni...

Descripción completa

Detalles Bibliográficos
Autores principales: Horne, Jim E., Radford, Sheena E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900752/
https://www.ncbi.nlm.nih.gov/pubmed/27284045
http://dx.doi.org/10.1042/BST20160020
_version_ 1782436699094122496
author Horne, Jim E.
Radford, Sheena E.
author_facet Horne, Jim E.
Radford, Sheena E.
author_sort Horne, Jim E.
collection PubMed
description Great strides into understanding protein folding have been made since the seminal work of Anfinsen over 40 years ago, but progress in the study of membrane protein folding has lagged behind that of their water soluble counterparts. Researchers in these fields continue to turn to more advanced techniques such as NMR, mass spectrometry, molecular dynamics (MD) and single molecule methods to interrogate how proteins fold. Our understanding of β-barrel outer membrane protein (OMP) folding has benefited from these advances in the last decade. This class of proteins must traverse the periplasm and then insert into an asymmetric lipid membrane in the absence of a chemical energy source. In this review we discuss old, new and emerging techniques used to examine the process of OMP folding and biogenesis in vitro and describe some of the insights and new questions these techniques have revealed.
format Online
Article
Text
id pubmed-4900752
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-49007522016-06-23 A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis Horne, Jim E. Radford, Sheena E. Biochem Soc Trans Biochemical Society Focused Meetings Great strides into understanding protein folding have been made since the seminal work of Anfinsen over 40 years ago, but progress in the study of membrane protein folding has lagged behind that of their water soluble counterparts. Researchers in these fields continue to turn to more advanced techniques such as NMR, mass spectrometry, molecular dynamics (MD) and single molecule methods to interrogate how proteins fold. Our understanding of β-barrel outer membrane protein (OMP) folding has benefited from these advances in the last decade. This class of proteins must traverse the periplasm and then insert into an asymmetric lipid membrane in the absence of a chemical energy source. In this review we discuss old, new and emerging techniques used to examine the process of OMP folding and biogenesis in vitro and describe some of the insights and new questions these techniques have revealed. Portland Press Ltd. 2016-06-09 2016-06-15 /pmc/articles/PMC4900752/ /pubmed/27284045 http://dx.doi.org/10.1042/BST20160020 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biochemical Society Focused Meetings
Horne, Jim E.
Radford, Sheena E.
A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
title A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
title_full A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
title_fullStr A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
title_full_unstemmed A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
title_short A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
title_sort growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis
topic Biochemical Society Focused Meetings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900752/
https://www.ncbi.nlm.nih.gov/pubmed/27284045
http://dx.doi.org/10.1042/BST20160020
work_keys_str_mv AT hornejime agrowingtoolboxoftechniquesforstudyingbbarreloutermembraneproteinfoldingandbiogenesis
AT radfordsheenae agrowingtoolboxoftechniquesforstudyingbbarreloutermembraneproteinfoldingandbiogenesis
AT hornejime growingtoolboxoftechniquesforstudyingbbarreloutermembraneproteinfoldingandbiogenesis
AT radfordsheenae growingtoolboxoftechniquesforstudyingbbarreloutermembraneproteinfoldingandbiogenesis