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Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...

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Detalles Bibliográficos
Autores principales:	Hussain, Rohanah, Harding, Stephen E., Hughes, Charlotte S., Ma, Pikyee, Patching, Simon G., Edara, Shalini, Siligardi, Giuliano, Henderson, Peter J.F., Phillips-Jones, Mary K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Portland Press Ltd. 2016
Materias:	Biochemical Society Focused Meetings
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900758/ https://www.ncbi.nlm.nih.gov/pubmed/27284046 http://dx.doi.org/10.1042/BST20160023

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