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Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study

The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. I...

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Autores principales: Ibrahim, Mohammad H. A., Lebbe, Liesbeth, Willems, Anne, Steinbüchel, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900968/
https://www.ncbi.nlm.nih.gov/pubmed/27277080
http://dx.doi.org/10.1186/s13568-016-0209-9
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author Ibrahim, Mohammad H. A.
Lebbe, Liesbeth
Willems, Anne
Steinbüchel, Alexander
author_facet Ibrahim, Mohammad H. A.
Lebbe, Liesbeth
Willems, Anne
Steinbüchel, Alexander
author_sort Ibrahim, Mohammad H. A.
collection PubMed
description The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum growth at 50 °C. Based on 16S rRNA gene sequence similarity, the strain together with five very similar isolates, was affiliated to the genus Chelatococcus (Ibrahim et al. in J Appl Microbiol 109:1579–1590, 2010). Rep-PCR genomic fingerprints and partial dnaK gene sequence also revealed that these isolates are very similar, but differ from other Chelatococcus type strains. The major fatty acids were similar to those of other strains of the genus Chelatococcus. DNA–DNA hybridization of strain MW9(T) with Chelatococcus species type strains revealed 11.0–47.7 % relatedness. G+C content of DNA was 67.1 mol%, which is comparable with the other strains of Chelatococcus species. The physiological and phenotypic characteristics of the new strain MW9(T) are sufficient to differentiate it from previously described species in the genus Chelatococcus. Strain MW9(T) is considered to represent a novel species of the genus Chelatococcus, for which the name Chelatococcus thermostellatus is proposed. The type strain is MW9(T) (=LMG 27009(T) = DSM 28244(T)). Compared to known Chelatococcus strains, strain MW9(T) could be a potent candidate for bioplastic production at elevated temperature.
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spelling pubmed-49009682016-06-27 Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study Ibrahim, Mohammad H. A. Lebbe, Liesbeth Willems, Anne Steinbüchel, Alexander AMB Express Original Article The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum growth at 50 °C. Based on 16S rRNA gene sequence similarity, the strain together with five very similar isolates, was affiliated to the genus Chelatococcus (Ibrahim et al. in J Appl Microbiol 109:1579–1590, 2010). Rep-PCR genomic fingerprints and partial dnaK gene sequence also revealed that these isolates are very similar, but differ from other Chelatococcus type strains. The major fatty acids were similar to those of other strains of the genus Chelatococcus. DNA–DNA hybridization of strain MW9(T) with Chelatococcus species type strains revealed 11.0–47.7 % relatedness. G+C content of DNA was 67.1 mol%, which is comparable with the other strains of Chelatococcus species. The physiological and phenotypic characteristics of the new strain MW9(T) are sufficient to differentiate it from previously described species in the genus Chelatococcus. Strain MW9(T) is considered to represent a novel species of the genus Chelatococcus, for which the name Chelatococcus thermostellatus is proposed. The type strain is MW9(T) (=LMG 27009(T) = DSM 28244(T)). Compared to known Chelatococcus strains, strain MW9(T) could be a potent candidate for bioplastic production at elevated temperature. Springer Berlin Heidelberg 2016-06-09 /pmc/articles/PMC4900968/ /pubmed/27277080 http://dx.doi.org/10.1186/s13568-016-0209-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Ibrahim, Mohammad H. A.
Lebbe, Liesbeth
Willems, Anne
Steinbüchel, Alexander
Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
title Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
title_full Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
title_fullStr Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
title_full_unstemmed Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
title_short Chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
title_sort chelatococcusthermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900968/
https://www.ncbi.nlm.nih.gov/pubmed/27277080
http://dx.doi.org/10.1186/s13568-016-0209-9
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