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Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901233/ https://www.ncbi.nlm.nih.gov/pubmed/27284142 http://dx.doi.org/10.1128/genomeA.00529-16 |
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author | Ohtsubo, Yoshiyuki Nonoyama, Shouta Nagata, Yuji Numata, Mitsuru Tsuchikane, Keiko Hosoyama, Akira Yamazoe, Atsushi Tsuda, Masataka Fujita, Nobuyuki Kawai, Fusako |
author_facet | Ohtsubo, Yoshiyuki Nonoyama, Shouta Nagata, Yuji Numata, Mitsuru Tsuchikane, Keiko Hosoyama, Akira Yamazoe, Atsushi Tsuda, Masataka Fujita, Nobuyuki Kawai, Fusako |
author_sort | Ohtsubo, Yoshiyuki |
collection | PubMed |
description | We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and the PacBio assembly was used as a reference. The PacBio assembly carried 414 nucleotide mismatches over 5,953,153 bases of the 203N genome. |
format | Online Article Text |
id | pubmed-4901233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49012332016-06-17 Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader Ohtsubo, Yoshiyuki Nonoyama, Shouta Nagata, Yuji Numata, Mitsuru Tsuchikane, Keiko Hosoyama, Akira Yamazoe, Atsushi Tsuda, Masataka Fujita, Nobuyuki Kawai, Fusako Genome Announc Prokaryotes We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and the PacBio assembly was used as a reference. The PacBio assembly carried 414 nucleotide mismatches over 5,953,153 bases of the 203N genome. American Society for Microbiology 2016-06-09 /pmc/articles/PMC4901233/ /pubmed/27284142 http://dx.doi.org/10.1128/genomeA.00529-16 Text en Copyright © 2016 Ohtsubo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Prokaryotes Ohtsubo, Yoshiyuki Nonoyama, Shouta Nagata, Yuji Numata, Mitsuru Tsuchikane, Keiko Hosoyama, Akira Yamazoe, Atsushi Tsuda, Masataka Fujita, Nobuyuki Kawai, Fusako Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader |
title | Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader |
title_full | Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader |
title_fullStr | Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader |
title_full_unstemmed | Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader |
title_short | Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader |
title_sort | complete genome sequence of sphingopyxis macrogoltabida strain 203n (nbrc 111659), a polyethylene glycol degrader |
topic | Prokaryotes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901233/ https://www.ncbi.nlm.nih.gov/pubmed/27284142 http://dx.doi.org/10.1128/genomeA.00529-16 |
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