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Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader

We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and...

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Autores principales: Ohtsubo, Yoshiyuki, Nonoyama, Shouta, Nagata, Yuji, Numata, Mitsuru, Tsuchikane, Keiko, Hosoyama, Akira, Yamazoe, Atsushi, Tsuda, Masataka, Fujita, Nobuyuki, Kawai, Fusako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901233/
https://www.ncbi.nlm.nih.gov/pubmed/27284142
http://dx.doi.org/10.1128/genomeA.00529-16
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author Ohtsubo, Yoshiyuki
Nonoyama, Shouta
Nagata, Yuji
Numata, Mitsuru
Tsuchikane, Keiko
Hosoyama, Akira
Yamazoe, Atsushi
Tsuda, Masataka
Fujita, Nobuyuki
Kawai, Fusako
author_facet Ohtsubo, Yoshiyuki
Nonoyama, Shouta
Nagata, Yuji
Numata, Mitsuru
Tsuchikane, Keiko
Hosoyama, Akira
Yamazoe, Atsushi
Tsuda, Masataka
Fujita, Nobuyuki
Kawai, Fusako
author_sort Ohtsubo, Yoshiyuki
collection PubMed
description We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and the PacBio assembly was used as a reference. The PacBio assembly carried 414 nucleotide mismatches over 5,953,153 bases of the 203N genome.
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spelling pubmed-49012332016-06-17 Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader Ohtsubo, Yoshiyuki Nonoyama, Shouta Nagata, Yuji Numata, Mitsuru Tsuchikane, Keiko Hosoyama, Akira Yamazoe, Atsushi Tsuda, Masataka Fujita, Nobuyuki Kawai, Fusako Genome Announc Prokaryotes We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and the PacBio assembly was used as a reference. The PacBio assembly carried 414 nucleotide mismatches over 5,953,153 bases of the 203N genome. American Society for Microbiology 2016-06-09 /pmc/articles/PMC4901233/ /pubmed/27284142 http://dx.doi.org/10.1128/genomeA.00529-16 Text en Copyright © 2016 Ohtsubo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Prokaryotes
Ohtsubo, Yoshiyuki
Nonoyama, Shouta
Nagata, Yuji
Numata, Mitsuru
Tsuchikane, Keiko
Hosoyama, Akira
Yamazoe, Atsushi
Tsuda, Masataka
Fujita, Nobuyuki
Kawai, Fusako
Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
title Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
title_full Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
title_fullStr Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
title_full_unstemmed Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
title_short Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
title_sort complete genome sequence of sphingopyxis macrogoltabida strain 203n (nbrc 111659), a polyethylene glycol degrader
topic Prokaryotes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901233/
https://www.ncbi.nlm.nih.gov/pubmed/27284142
http://dx.doi.org/10.1128/genomeA.00529-16
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