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Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers

The ALPK1 gene located in the 4q25 region encodes a newly explored protein kinase which could phosphorylate the amino acid of a domain full of α-helices. Recently, several studies have indicated that the expression of ALPK1 is related to inflammation and various diseases; therefore, the purpose of t...

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Autores principales: Liao, Hsien-Feng, Lee, Hsien-Hsiung, Chang, Ya-Sian, Lin, Chia-Li, Liu, Ting-Yuan, Chen, Yu-Chia, Yen, Ju-Chen, Lee, Ya-Ting, Lin, Chien-Yu, Wu, Shih-Hsiung, Ko, Ying-Chin, Chang, Jan-Gowth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901299/
https://www.ncbi.nlm.nih.gov/pubmed/27283888
http://dx.doi.org/10.1038/srep27350
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author Liao, Hsien-Feng
Lee, Hsien-Hsiung
Chang, Ya-Sian
Lin, Chia-Li
Liu, Ting-Yuan
Chen, Yu-Chia
Yen, Ju-Chen
Lee, Ya-Ting
Lin, Chien-Yu
Wu, Shih-Hsiung
Ko, Ying-Chin
Chang, Jan-Gowth
author_facet Liao, Hsien-Feng
Lee, Hsien-Hsiung
Chang, Ya-Sian
Lin, Chia-Li
Liu, Ting-Yuan
Chen, Yu-Chia
Yen, Ju-Chen
Lee, Ya-Ting
Lin, Chien-Yu
Wu, Shih-Hsiung
Ko, Ying-Chin
Chang, Jan-Gowth
author_sort Liao, Hsien-Feng
collection PubMed
description The ALPK1 gene located in the 4q25 region encodes a newly explored protein kinase which could phosphorylate the amino acid of a domain full of α-helices. Recently, several studies have indicated that the expression of ALPK1 is related to inflammation and various diseases; therefore, the purpose of this investigation was to determine whether the expression of ALPK1 has an influence on tumorigenesis and to further scrutinize its gene polymorphism in order to better understand its clinical importance. In lung and colorectal cancer tissues, the ALPK1 RNA level of the normal part was higher than that of the tumor part using the RT-qPCR analysis. Moreover, differences in HRM melting curves could effectively separate the known mutation sites and be used to identify the two novel variants that might cause the bio-dysfunctions of ALPK1 found in silico predictions. Additionally, in both Lovo colorectal and A549 lung cancer cells with enhanced and depleted expression of ALPK1, the encoded ALPK1 could exert its activity on cell migration without interfering with cell viability. Taken together, these findings suggested that ALPK1 might play a vital role in cancer development and that the newly explored SNPs are found in a Taiwanese cohort.
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spelling pubmed-49012992016-06-13 Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers Liao, Hsien-Feng Lee, Hsien-Hsiung Chang, Ya-Sian Lin, Chia-Li Liu, Ting-Yuan Chen, Yu-Chia Yen, Ju-Chen Lee, Ya-Ting Lin, Chien-Yu Wu, Shih-Hsiung Ko, Ying-Chin Chang, Jan-Gowth Sci Rep Article The ALPK1 gene located in the 4q25 region encodes a newly explored protein kinase which could phosphorylate the amino acid of a domain full of α-helices. Recently, several studies have indicated that the expression of ALPK1 is related to inflammation and various diseases; therefore, the purpose of this investigation was to determine whether the expression of ALPK1 has an influence on tumorigenesis and to further scrutinize its gene polymorphism in order to better understand its clinical importance. In lung and colorectal cancer tissues, the ALPK1 RNA level of the normal part was higher than that of the tumor part using the RT-qPCR analysis. Moreover, differences in HRM melting curves could effectively separate the known mutation sites and be used to identify the two novel variants that might cause the bio-dysfunctions of ALPK1 found in silico predictions. Additionally, in both Lovo colorectal and A549 lung cancer cells with enhanced and depleted expression of ALPK1, the encoded ALPK1 could exert its activity on cell migration without interfering with cell viability. Taken together, these findings suggested that ALPK1 might play a vital role in cancer development and that the newly explored SNPs are found in a Taiwanese cohort. Nature Publishing Group 2016-06-10 /pmc/articles/PMC4901299/ /pubmed/27283888 http://dx.doi.org/10.1038/srep27350 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liao, Hsien-Feng
Lee, Hsien-Hsiung
Chang, Ya-Sian
Lin, Chia-Li
Liu, Ting-Yuan
Chen, Yu-Chia
Yen, Ju-Chen
Lee, Ya-Ting
Lin, Chien-Yu
Wu, Shih-Hsiung
Ko, Ying-Chin
Chang, Jan-Gowth
Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers
title Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers
title_full Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers
title_fullStr Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers
title_full_unstemmed Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers
title_short Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers
title_sort down-regulated and commonly mutated alpk1 in lung and colorectal cancers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901299/
https://www.ncbi.nlm.nih.gov/pubmed/27283888
http://dx.doi.org/10.1038/srep27350
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